Rat monoamine oxidase(MAO) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠单胺氧化酶(MAO)酶联免疫试剂盒（CSB-E15076r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MAO含量。MAO（单胺氧化酶）是一种催化单胺类物质氧化脱氨反应的酶，存在于细胞线粒体外膜上，广泛分布于人体肝、脑、肾等组织细胞内。其研究机制涉及酶的结构、活性位点、催化过程等，对理解生物体内胺浓度调节及相关疾病有重要意义。试剂盒检测范围为93.75 mU/mL-6000 mU/mL，适用于神经科学、药理学等领域的基础研究，例如探究药物干预或疾病模型下MAO活性变化、评估神经递质代谢调控机制等科研场景，为探索神经系统相关疾病的分子机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Maoa; Amine oxidase [flavin-containing] A; Monoamine oxidase type A; MAO-A
缩写：

MAO
Uniprot No.：

P21396
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

93.75 mU/mL-6000 mU/mL
灵敏度：

23.43 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	88-96
1:10	Average %	96
	Range %	90-102
1:20	Average %	102
	Range %	98-104
1:40	Average %	87
	Range %	83-92

回收率：

The recovery of rat MAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	89	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.
亚细胞定位：

Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
蛋白家族：

Flavin monoamine oxidase family
数据库链接：

STRING: 10116.ENSRNOP00000063784

UniGene: Rn.224544