Rat matrix metalloproteinase 3,MMP-3 ELISA kit

Rat matrix metalloproteinase 3,MMP-3 ELISA kit

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中文名称：

大鼠基质金属蛋白酶3(MMP-3)酶联免疫试剂盒
货号：

CSB-E07410r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO专为大鼠样本设计的基质金属蛋白酶3（MMP-3）酶联免疫检测试剂盒（货号：CSB-E07410r），采用双抗体夹心法原理实现定量分析，适用于血清、血浆、细胞培养上清及组织匀浆等多种样本类型。MMP-3作为基质金属蛋白酶家族成员，在细胞外基质降解、组织修复及炎症反应中发挥关键作用，其异常表达与关节炎、肿瘤侵袭等病理过程密切相关。试剂盒检测灵敏度达1.56 pg/mL，线性范围为1.56-100 pg/mL，通过预包被特异性捕获抗体与标记检测抗体的协同作用，精准检测目标蛋白浓度。本产品可广泛应用于基础医学研究领域，如关节炎动物模型中的基质代谢研究、药物干预下MMP-3表达水平动态监测，或肿瘤微环境中蛋白酶活性评估等实验场景，为组织重塑机制探索及病理模型构建提供可靠工具。实验操作兼容常规酶标仪检测系统，配套标准品及优化缓冲液确保检测重复性，所有组分均经严格质控验证。
别名：

Mmp3 ELISA Kit; Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit; PTR1 protein ELISA Kit; Transin-1 ELISA Kit
缩写：

MMP3
Uniprot No.：

P03957
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	90
	Range %	87-93
1:200	Average %	94
	Range %	90-98
1:400	Average %	95
	Range %	91-99
1:800	Average %	92
	Range %	88-96

回收率：

The recovery of rat MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	94	90-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
100	2.569	2.547	2.558	2.429
50	2.236	2.213	2.225	2.096
25	1.868	1.829	1.849	1.720
12.5	1.367	1.387	1.377	1.248
6.25	0.840	0.889	0.865	0.736
3.13	0.377	0.387	0.382	0.253
1.56	0.247	0.259	0.253	0.124
0	0.128	0.130	0.129

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Matrix metalloproteinases are possible targets inmonocrotaline-induced pulmonary hypertension: investigationof anti-remodeling effects of alagebrium and everolimus Atl.et al,Anatolian journal of cardiology,2016

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功能：

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
亚细胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
数据库链接：

UniGene: Rn.32086

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.