Rat matrix metalloproteinase 13,MMP-13 ELISA kit

Rat matrix metalloproteinase 13,MMP-13 ELISA kit

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中文名称：

大鼠基质金属蛋白酶13(MMP-13)酶联免疫试剂盒
货号：

CSB-E07412r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠基质金属蛋白酶13（MMP-13）酶联免疫检测试剂盒（货号：CSB-E07412r），采用双抗体夹心法定量分析血清、血浆及组织匀浆样本中的MMP-13含量。MMP-13是基质金属蛋白酶家族的重要成员，可特异性降解II型胶原蛋白，在组织重塑、炎症反应及病理状态下的细胞外基质降解过程中发挥关键作用。本试剂盒检测灵敏度高，线性范围覆盖93.75-6000 pg/mL，可精准检测不同浓度水平的MMP-13表达变化，适用于探索骨关节炎、肿瘤侵袭转移等疾病模型中基质代谢调控机制，或评估创伤修复、炎症微环境等基础研究中的蛋白酶活性动态。实验操作采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配套提供标准品、显色底物及终止液，支持科研人员高效完成细胞培养上清、动物模型体液或组织样本的定量分析。
别名：

Mmp13 ELISA Kit; Collagenase 3 ELISA Kit; EC 3.4.24.- ELISA Kit; Matrix metalloproteinase-13 ELISA Kit; MMP-13 ELISA Kit; UMRCASE ELISA Kit; Fragment ELISA Kit
缩写：

MMP13
Uniprot No.：

P23097
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

93.75 pg/mL-6000 pg/mL
灵敏度：

23.44 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	83-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	98
	Range %	93-104
1:8	Average %	102
	Range %	98-105

回收率：

The recovery of rat MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	90	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
6000	2.231	2.269	2.250	2.138
3000	1.526	1.539	1.533	1.421
1500	0.974	0.997	0.986	0.874
750	0.578	0.596	0.587	0.475
375	0.345	0.327	0.336	0.224
187.5	0.251	0.241	0.246	0.134
93.75	0.185	0.189	0.187	0.075
0	0.111	0.113	0.112

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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SERUM CONCENTRATIONS OF MATRIX METALLOPROTEINASE-9,-13 AND TIMP-1 IN AN OVARIECTOMIZED WISTAR RAT MODEL OF OSTEOPOROSIS Armine V. Grigoryan.et al,Archives of the Balkan Medical Union,2017
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Advanced Oxidation Protein Products Induce Inflammatory Response in Fibroblast-Like Synoviocytes through NADPH Oxidase-Dependent Activation of NF-kB Zheng S et al,Cell Physiol Biochem,2013
Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans Franchi M et al,Connect Tissue Res,2013

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功能：

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
亚细胞定位：

Secreted, extracellular space, extracellular matrix. Secreted.
蛋白家族：

Peptidase M10A family
数据库链接：

STRING: 10116.ENSRNOP00000011507

UniGene: Rn.10997

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.