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中文名称：大鼠基质金属蛋白酶10(MMP-10)酶联免疫试剂盒
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货号：CSB-E07414r
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠基质金属蛋白酶10（MMP-10）酶联免疫检测试剂盒（货号：CSB-E07414r），采用竞争法原理实现定量分析，适用于血清、血浆及组织匀浆样本中MMP-10的精准检测。MMP-10作为基质金属蛋白酶家族成员，在细胞外基质重塑、炎症调控及组织修复等生理病理过程中发挥重要作用，其表达水平与肿瘤微环境、纤维化疾病等研究密切相关。本试剂盒检测范围为80-2000 pg/mL，最低检测限为80 pg/mL，操作流程约3小时，通过预包被96孔板中的特异性抗体与样本中MMP-10及标记物竞争结合，结合酶标二抗显色后，经酶标仪测定吸光度（OD值），最终通过标准曲线计算目标物浓度。建议实验前对血浆/血清样本进行离心去沉淀处理，组织样本需制备匀浆并经低速离心取上清液后检测。该产品可广泛应用于炎症性疾病机制探究、肿瘤转移模型分析，以及心血管疾病或肝纤维化等科研领域的基础研究，为探索MMP-10的生物学功能提供可靠的检测工具。
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别名：Mmp10 ELISA Kit; Stromelysin-2 ELISA Kit; SL-2 ELISA Kit; EC 3.4.24.22 ELISA Kit; Matrix metalloproteinase-10 ELISA Kit; MMP-10 ELISA Kit; Transformation-associated protein 34A ELISA Kit; Transin-2 ELISA Kit
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缩写：MMP10
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Uniprot No.：
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：80 pg/mL-2000 pg/mL
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灵敏度：50 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Cancer
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测定原理：quantitative
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测定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 85-99 1:2 Average % 92 Range % 90-103 1:4 Average % 95 Range % 87-105 1:8 Average % 95 Range % 87-99 -
回收率：
The recovery of rat MMP-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 88-100 EDTA plasma (n=4) 95 89-102 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 2000 0.172 0.175 0.174 800 0.376 0.379 0.378 480 0.554 0.562 0.558 200 0.882 0.897 0.890 80 1.493 1.475 1.484 0 2.404 2.452 2.428 -
数据处理：
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货期：3-5 working days
引用文献
- Procyanidin B2 ameliorates the progression of osteoarthritis: An in vitro and in vivo study W Cai,International Immunopharmacology,2022
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靶点详情
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功能：Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
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基因功能参考文献：
- MMP-10 modulates corneal epithelial wound healing. PMID: 24339723
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亚细胞定位：Secreted, extracellular space, extracellular matrix.
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蛋白家族：Peptidase M10A family
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数据库链接：
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