Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

Rat matrix metalloproteinase 1(MMP-1/Collagenase I) ELISA kit

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中文名称：

大鼠基质金属蛋白酶1(MMP-1/Collagenase I)酶联免疫试剂盒
货号：

CSB-E07416r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠基质金属蛋白酶1（MMP-1/Collagenase I）定量检测试剂盒（货号：CSB-E07416r），采用双抗体夹心法原理设计，适用于科研领域对血清、血浆及组织匀浆样本中MMP-1蛋白含量的精准分析。MMP-1作为胶原酶家族成员，在细胞外基质降解、组织重塑及炎症反应中发挥关键作用，其表达水平与纤维化疾病、创伤修复等病理生理过程密切相关。本试剂盒检测灵敏度达0.235 ng/ml，线性范围为0.235-15 ng/ml，可覆盖大鼠生物样本中MMP-1的基础浓度至病理状态下的高表达水平。实验操作基于预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，支持科研人员高效完成样本中MMP-1的定量检测。该试剂盒适用于炎症模型、组织工程研究、纤维化疾病机制探索等科研场景，能够为心血管疾病、关节炎等动物模型的分子机制研究提供可靠数据支持，所有检测步骤均经过严格验证，确保结果的稳定性和重复性。
别名：

Mmp1Interstitial collagenase ELISA Kit; EC 3.4.24.7 ELISA Kit; Fibroblast collagenase ELISA Kit; Matrix metalloproteinase-1 ELISA Kit; MMP-1 ELISA Kit; Myocardial collagenase ELISA Kit; Fragment ELISA Kit
缩写：

MMP1
Uniprot No.：

P81563
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.235 ng/ml - 15 ng/ml
灵敏度：

0.058 ng/ml
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-1/Collagenase I in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	92
	Range %	87-98
1:4	Average %	95
	Range %	90-102
1:8	Average %	100
	Range %	96-109

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.257

2.231

2.244

2.147

7.5

1.876

1.832

1.854

1.757

3.75

1.381

1.373

1.377

1.280

1.88

0.835

0.797

0.816

0.719

0.94

0.397

0.376

0.387

0.290

0.47

0.273

0.257

0.265

0.168

0.235

0.197

0.207

0.202

0.105

0.099

0.094

0.097

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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Dietary Supplementation with Palmitoyl-Glucosamine Co-Micronized with Curcumin Relieves Osteoarthritis Pain and Benefits Joint Mobility E Gugliandolo,Animals,2020
Ethanolic and Aqueous Extracts of Avocado (Persea americana) Seeds Attenuates Doxorubicin-Induced Cardiotoxicity in Male Albino Rats G Shamlan,Arabian Journal for Science and Engineering,2020
Safety and efficacy of a new micronized formulation of the ALIAmide palmitoylglucosamine in preclinical models of inflammation and osteoarthritis pain Cordaro M, et al,Arthritis Research & Therapy,2019
A novel composite formulation of palmitoylethanolamide and quercetin decreases inflammation and relieves pain in inflammatory and osteoarthritic pain models Britti D.et al,BMC Vet Res.,2017
Adelmidrol, in combination with hyaluronic acid, displays increased anti-inflammatory and analgesic effects against monosodium iodoacetate-induced osteoarthritis in rats Rosanna Di Paola.et al,Arthritis Research & Therapy,2016
Matrix metalloproteinases are possible targets inmonocrotaline-induced pulmonary hypertension: investigationof anti-remodeling effects of alagebrium and everolimus Atl.et al,Anatolian journal of cardiology,2016

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最新研究进展：

MMP1，全名为基质金属蛋白酶-1，是一种广泛存在于人体组织中的酶类分子。MMP1主要参与胶原蛋白的降解和清除过程，在组织再生、炎症、肿瘤转移等多种生理和病理过程中发挥重要作用。最近的研究表明，MMP1在多种癌症的发生和发展中发挥关键作用，成为了一个重要的治疗靶点。此外，MMP1还与关节炎、动脉粥样硬化等疾病的发生和发展密切相关，成为了重要的生物标志物和疾病预测因子。针对这些酶的抑制剂已成为研究的热点，可以用于疾病的预防和治疗。
功能：

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. May play a role in the deterioration of the heart wall extracellular matrix proteins during the onset of dilated cardiomyopathy.
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
数据库链接：

UniGene: Rn.79007

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.