-
中文名称：大鼠巨噬细胞炎性蛋白2(MIP-2)酶联免疫试剂盒
-
货号：CSB-E07419r
-
规格：96T/48T
-
价格：￥3600/￥2500
-
其他：
产品详情
-
产品描述：CUSABIO大鼠巨噬细胞炎性蛋白2（MIP-2/CXCL2）酶联免疫检测试剂盒（货号：CSB-E07419r），采用双抗体夹心法原理实现定量分析，可检测血清、血浆及组织匀浆样本中MIP-2的蛋白表达水平，检测灵敏度达78 pg/mL，线性范围覆盖78 pg/mL至5000 pg/mL。MIP-2作为CXC趋化因子家族成员，在炎症反应中通过激活CXCR2受体介导中性粒细胞迁移，广泛参与感染、创伤修复及免疫性疾病等病理生理过程。本试剂盒具有高特异性和稳定性，实验操作全程可在4-6小时内完成，适用于科研领域中对炎症机制、药物干预效果及疾病动物模型的研究，例如通过检测组织匀浆中的MIP-2水平评估局部炎症程度，或分析血液样本中趋化因子动态变化以探索全身性炎症反应的调控通路。试剂盒配套提供标准品、预包被抗体及优化缓冲体系，支持研究者精准量化大鼠样本中的MIP-2浓度，为炎症相关基础研究提供可靠工具。
-
别名：Cxcl2 ELISA kit; Cinc3 ELISA kit; Mip-2 ELISA kit; Mip2 ELISA kit; Scyb2C-X-C motif chemokine 2 ELISA kit; Cytokine-induced neutrophil chemoattractant 3 ELISA kit; CINC-3 ELISA kit; Macrophage inflammatory protein 2 ELISA kit; MIP2 ELISA kit
-
缩写：
-
Uniprot No.：
-
种属：Rattus norvegicus (Rat)
-
样本类型：serum, plasma, tissue homogenates
-
检测范围：78 pg/mL-5000 pg/mL
-
灵敏度：19.5 pg/mL
-
反应时间：1-5h
-
样本体积：50-100ul
-
检测波长：450 nm
-
研究领域：Immunology
-
测定原理：quantitative
-
测定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
-
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
88
Range %
80-95
1:2
Average %
90
Range %
85-99
1:4
Average %
98
Range %
94-101
1:8
Average %
93
Range %
86-98
-
回收率：
The recovery of rat MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
95
89-98
EDTA plasma (n=4)
89
84-97
-
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
5000
2.899
2.934
2.916
2.778
2500
2.437
2.567
2.502
2.364
1250
1.817
1.828
1.822
1.684
625
1.228
1.229
1.229
1.091
312
0.736
0.721
0.729
0.591
156
0.445
0.453
0.449
0.311
78
0.263
0.254
0.259
0.121
0
0.141
0.135
0.138
-
数据处理：
-
货期：3-5 working days
引用文献
- Gut Microbial Metabolite Trimethylamine N-Oxide Aggravates Pulmonary W Lu,American journal of respiratory cell and molecular biology,2022
- Electroacupuncture Prevents Osteoarthritis of High-Fat Diet-Induced Obese Rats X Lin-Lin,BIOMED RESEARCH INTERNATIONAL,2020
- Annexin V homodimer protects against ischemia reperfusion-induced acute lung injury in lung transplantation Hashimoto K.et al,J Thorac Cardiovasc Surg.,2016
- Endotoxin-induced lung alveolar cell injury causes brain cell damage Rodr铆guez-Gonz谩lez R.et al,Exp Biol Med (Maywood).,2015
- Annexin V Homo-dimer Protects against Ischemia Reperfusion-Induced Acute Lung Injury in Lung Transplantation Kohei Hashimoto. et al,The Journal of Thoracic and Cardiovascular Surgery,2015
产品评价
相关产品
靶点详情
-
最新研究进展：CXCL2是介导中性粒细胞、嗜酸性粒细胞和巨噬细胞趋化的重要化学趋化因子。最新研究表明，CXCL2在许多炎症疾病中起重要作用，如哮喘、炎性肠病、骨关节炎等。此外，CXCL2还参与了肿瘤发生、发展和转移过程，是潜在的抗肿瘤治疗靶点。
-
功能：Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
-
基因功能参考文献：
- this study shows elevated expression of CINC-3 at the site of inflammation as well as their significant reflection in the circulation, thereby suggesting their frontline role in carrageenan-induced acute inflammation PMID: 27967265
- rutin is a potential protective agent in spinal cord injury enhancing the neurotrophic effect by inhibiting the expression of MIP-2 and activation of MMP-9, and downregulating the expression of p-Akt. PMID: 26502930
- The rat spiral ligament fibrocytes were found to release CXCL2 in response to nontypeable H. influenzae via activation of c-Jun, leading to the recruitment of polymorphonuclear cells to the cochlea. PMID: 22379036
- CsA has no significant effect on serum levels of MCP-1 and MIP-2 following renal transplantation in rats. PMID: 20034917
- Results identify FQHPSFI peptide may be used for the modulation of lipopolysaccharide-stimulated MIP-2 production in alveolar macrophagess. PMID: 20186460
- chemokine production by rat alveolar macrophages is inhibited by taurine chloramine PMID: 11716962
- Intratracheal LPS induced a significant increase in MIP-2 in bronchoalveolar lavage (BAL) fluid with no detectable MIP-2 in the plasma. PMID: 11837784
- demonstrated that the beta-glucan component of the Pneumocystis carinii cell wall is able to stimulate alveolar epithelial cells to produce MIP-2 PMID: 11906040
- role for the nitric oxide pathway in the overproduction of pro-inflammatory mediators IL-6 and MIP-2 during GBS-induced lung inflammation. PMID: 12661899
- junctional epithelium cells produced MIP-2 and CINC-2 in response to LPS stimulation, suggesting that MIP-2 and CINC-2 may be responsible for PMN migration toward the periodontal pathogen and may play an important role in the initiation of inflammation PMID: 15045510
- macrophage inflammatory protein 2 mediates platelet-activating factor-induced intestinal injury PMID: 15319184
- no significant differences were detected for MIP-2 levels in diabetic rats when compared with non-diabetic rats after hypoxia PMID: 16132691
- Following cecal ligation and puncture Cxcl2 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
- MIP-2/CXCL2 and MCP-1/CCL2 are increased after injury, and neurons appear to be the source of this expression. Chemokine expression was selectively inhibited by dexamethasone. PMID: 19210118
显示更多
收起更多
-
亚细胞定位：Secreted.
-
蛋白家族：Intercrine alpha (chemokine CxC) family
-
组织特异性：At least expressed in the lung and trachea.
-
数据库链接：
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
