Rat lipoprotein-associated phospholipase A2,Lp-PLA2 ELISA Kit

产品详情

产品描述：

大鼠脂蛋白磷脂酶A2(Lp-PLA2)酶联免疫试剂盒（CSB-E08320r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Lp-PLA2含量。Lp-PLA2（脂蛋白相关磷脂酶A2）是一种与动脉粥样硬化和心血管疾病密切相关的标志物，由血管内膜中的巨噬细胞、T细胞和肥大细胞分泌。其水平高低与脑卒中和心血管疾病风险密切相关，通过水解氧化低密度脂蛋白中的氧化磷脂，生成脂类促炎物质，促进动脉粥样硬化。试剂盒检测范围为0.78 IU/mL-50 IU/mL，该产品适用于科研领域中对大鼠模型血清或血浆样本的Lp-PLA2水平监测，例如心血管疾病动物模型的病理机制研究、药物干预效果评估或炎症相关代谢调控实验，为体外实验提供可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

Lp-PLA2
Uniprot No.：

Q5M7T7
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

0.78 IU/mL-50 IU/mL
灵敏度：

0.195 IU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Lp-PLA2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	105
	Range %	100-110
1:4	Average %	86
	Range %	82-90
1:8	Average %	93
	Range %	89-98

回收率：

The recovery of rat Lp-PLA2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	88	84-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.