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中文名称：大鼠白三烯D4(LTD4)酶联免疫试剂盒
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货号：CSB-EQ027312RA
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠白三烯D4（LTD4）酶联免疫试剂盒（货号：CSB-EQ027312RA），采用夹心法原理进行定量检测，适用于分析血清、血浆或细胞培养上清液样本中的LTD4浓度。LTD4作为一种重要的炎症介质，广泛参与哮喘、过敏反应及炎症性疾病的发生发展，相关研究对解析炎症调控机制具有重要意义。试剂盒检测范围为7.8-500 pg/mL，灵敏度达7.8 pg/mL，样本需预先离心去除颗粒物以保证检测准确性。实验采用96孔板预包被特异性抗体，通过标准曲线定量目标物浓度，基于双抗体夹心法原理，样本中的LTD4依次与捕获抗体、生物素标记检测抗体结合，最终通过链霉亲和素-HRP催化底物显色，测量OD值完成定量分析。试剂盒包含预包被微孔板、LTD4标准品、检测抗体、显色底物及终止液等核心组分，标准品与检测抗体需-20℃保存，其余组分可短期存放于2-8℃，整体保质期为6个月。本产品适用于基础科研中LTD4相关通路研究，如呼吸道疾病动物模型评估、炎症信号机制探索或抗炎药物筛选等实验场景，为体外实验提供稳定可靠的数据支持。
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缩写：LTD4
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, cell culture supernates
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检测范围：7.8 pg/mL-500 pg/mL
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灵敏度：1.95 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Biochemicals
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat LTD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-97 1:2 Average % 103 Range % 99-108 1:4 Average % 91 Range % 85-97 1:8 Average % 94 Range % 91-98 -
回收率：
The recovery of rat LTD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 92-101 EDTA plasma (n=4) 92 87-98 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 500 2.843 2.960 2.902 2.799 250 2.388 2.324 2.356 2.253 125 1.573 1.548 1.561 1.458 62.5 0.872 0.884 0.878 0.775 31.2 0.445 0.438 0.442 0.339 15.6 0.323 0.310 0.317 0.214 7.8 0.215 0.206 0.211 0.108 0 0.104 0.102 0.103 -
数据处理：
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货期：3-5 working days
引用文献
- Yan-Hou-Qing formula attenuates ammonia-induced acute pharyngitis in rats via inhibition of NF-κB and COX-2 M Xu,BMC Complementary Medicine and Therapies,2020
- Enhanced lipoxygenase/LTD4 signaling accounts for the exaggerated hypertensive and nephrotoxic effects of cyclosporine plus indomethacin in rats Helmy MW.et al,Biomedicine & Pharmacotherapy,2018
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