Rat leukemia inhibitory factor,LIF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO双抗体夹心法ELISA试剂盒，专用于定量检测大鼠血清、血浆、支气管肺泡灌洗液及组织匀浆样本中的白血病抑制因子(LIF)水平。LIF作为多功能细胞因子，在胚胎发育、干细胞多能性维持及炎症调控中发挥重要作用，其表达变化与肺部疾病、组织修复等病理生理过程密切相关。试剂盒采用高灵敏度检测体系，覆盖0.312-20 ng/mL的宽检测范围，可精准捕捉低丰度样本中的LIF浓度变化。预包被特异性抗体与生物素标记检测抗体的双重结合保障检测特异性，配套优化的标准品体系确保定量准确性。该试剂盒适用于呼吸系统疾病模型研究、干细胞分化调控实验及炎症相关机制探索，可为肺纤维化、急性肺损伤等动物模型中的LIF动态监测提供可靠工具，助力科研人员深入解析LIF在组织再生、免疫微环境调节中的生物学功能。
别名：

LifLeukemia inhibitory factor ELISA Kit; LIF ELISA Kit; Cholinergic neuronal differentiation factor ELISA Kit
缩写：

LIF
Uniprot No.：

P17777
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, bronchoalveolar lavage fluid, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat LIF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	93
	Range %	89-97
1:4	Average %	99
	Range %	93-104
1:8	Average %	90
	Range %	86-98

回收率：

The recovery of rat LIF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-92
EDTA plasma (n=4)	95	89-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
亚细胞定位：

Secreted.
蛋白家族：

LIF/OSM family
数据库链接：

STRING: 10116.ENSRNOP00000009313

UniGene: Rn.44379