Rat inhibin B,INH-B ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠抑制素B（INH-B）酶联免疫试剂盒（货号：CSB-E08150r），采用基于竞争法的定量检测技术，适用于血清、血浆及组织匀浆样本中INH-B的精确测定，检测灵敏度范围为1-200 pg/mL。抑制素B是由睾丸支持细胞和卵巢颗粒细胞分泌的糖蛋白激素，属于转化生长因子β超家族成员，通过负反馈调节促卵泡激素（FSH）的合成与分泌，在生殖系统发育、性腺功能调控及生殖细胞成熟等生理过程中发挥关键作用。本试剂盒通过高特异性抗体与样本中INH-B的竞争性结合反应实现定量分析，可广泛应用于生殖生物学研究、激素调控机制探索，以及生殖相关疾病动物模型（如睾丸功能障碍、多囊卵巢综合征等）的科研分析。其检测体系经过严格优化，能够稳定检测低浓度样本，为科研人员提供可靠的数据支持，适用于实验室中对大鼠模型生殖内分泌状态的基础研究与病理机制评估。
别名：

N/A
缩写：

INH-B
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1 pg/mL-200 pg/mL
灵敏度：

0.5 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat INH-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-106
1:2	Average %	98
	Range %	90-105
1:4	Average %	94
	Range %	89-99
1:8	Average %	96
	Range %	90-100

回收率：

The recovery of rat INH-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	87-104
EDTA plasma (n=4)	97	91-106