Rat heat shock protein,HSP47 ELISA Kit

Rat heat shock protein,HSP47 ELISA Kit

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中文名称：

大鼠热休克蛋白47(HSP47)酶联免疫试剂盒
货号：

CSB-E11328r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠热休克蛋白47(HSP47)酶联免疫试剂盒（CSB-E11328r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SERPINH1含量。SERPINH1是一个具有重要研究价值的靶点。它在细胞内参与蛋白质折叠、稳定等过程。目前研究聚焦其在疾病发生发展中的机制，如肿瘤进展、纤维化等病理进程，解析其作用机制或能为开发相关治疗药物、改善治疗方案提供新方向。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于科研领域中对大鼠模型胶原代谢相关机制的研究，例如肝/肺纤维化疾病模型的分子机制探索、皮肤或骨骼损伤修复过程中胶原沉积的动态分析，以及药物干预下HSP47表达水平变化的评估。试剂盒兼容多类型生物样本，可为体外实验或动物模型研究提供高灵敏度的检测方案，助力科研人员高效获取HSP47在胶原相关通路中的调控数据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Serpinh1 ELISA Kit; Cbp1 ELISA Kit; Hsp47 ELISA Kit; Serpin H1 ELISA Kit; 47 kDa heat shock protein ELISA Kit; Collagen-binding protein ELISA Kit; Colligin ELISA Kit; GP46 ELISA Kit
缩写：

SERPINH1
Uniprot No.：

P29457
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP47 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-93
1:2	Average %	102
	Range %	98-105
1:4	Average %	92
	Range %	88-96
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of rat HSP47 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	87	83-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.311	2.507	2.409	2.261
5	1.740	1.645	1.693	1.545
2.5	1.087	1.124	1.106	0.958
1.25	0.664	0.652	0.658	0.510
0.625	0.420	0.433	0.427	0.279
0.312	0.339	0.327	0.333	0.185
0.156	0.226	0.230	0.228	0.080
0	0.147	0.148	0.148

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
基因功能参考文献：
1. The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production. PMID: 25526199
2. miR-29b down-regulates heat shock protein 47 and LOX expression. PMID: 24650661
3. HSP47 specifically modulates TGF-beta1-induced extracellular matrix protein synthesis in HK-2 cells PMID: 22718885
4. Heat shock protein 47 (HSP47) antisense oligonucleotides reduce cardiac remodeling and improve cardiac function in a rat model of myocardial infarction. PMID: 21412710
5. Involvement of HSP47 in progression of peritoneal fibrosis. Inhibition of HSP47 expression might merit further clinical investigation for treatment of peritoneal fibrosis in continuous ambulatory peritoneal dialysis. PMID: 12911538
6. Microgravity differentially modulated the expression of the molecular chaperone HSP47 in osteoblasts PMID: 15033773
7. expression of HSP47, a collagen-specific molecular chaperone, responds to gravitational changes, including microgravity and hypergravity in vitro and in vivo PMID: 17054723
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亚细胞定位：

Endoplasmic reticulum lumen.
蛋白家族：

Serpin family
数据库链接：

KEGG: rno:29345

STRING: 10116.ENSRNOP00000022983

UniGene: Rn.98199

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.