Rat heat shock protein 27,HSP-27 ELISA Kit

中文名称：

大鼠热休克蛋白27(HSP-27)酶联免疫试剂盒
货号：

CSB-E09240r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
引用文献
相关产品
靶点详情

产品详情

产品描述：

CUSABIO专为大鼠样本设计的热休克蛋白27（HSP-27）定量检测试剂盒（货号：CSB-E09240r），采用双抗体夹心法原理，可精准检测血清、血浆及组织匀浆中的HSP-27蛋白，检测范围为0.78-50 ng/mL，灵敏度达0.195 ng/mL。HSP-27属于小分子热休克蛋白家族，在细胞应激保护、抗氧化及抗凋亡过程中发挥关键作用，其表达水平与氧化应激、炎症反应及细胞损伤修复等生物学过程密切相关。该试剂盒通过预包被特异性抗体捕获目标蛋白，结合生物素标记的二抗及链霉亲和素-HRP系统实现信号放大，最终通过显色反应进行定量分析，操作流程高效稳定。适用于科研领域中对大鼠模型在热应激、神经退行性疾病、代谢性疾病或组织损伤修复等实验条件下的HSP-27动态变化研究，为探索其分子机制及调控网络提供可靠工具。试剂盒配套标准品及优化缓冲液体系，支持多种生物样本的高重复性检测。
别名：

Hspb1 ELISA Kit; Hsp27 ELISA Kit; Heat shock protein beta-1 ELISA Kit; HspB1 ELISA Kit; Heat shock 27 kDa protein ELISA Kit; HSP 27 ELISA Kit
缩写：

HSPB1
Uniprot No.：

P42930
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.78 ng/mL-50 ng/mL
灵敏度：

0.195 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat HSP-27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	93-102
1:2	Average %	105
	Range %	100-113
1:4	Average %	91
	Range %	85-96
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of rat HSP-27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-93
EDTA plasma (n=4)	85	80-90

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
50	2.361	2.293	2.327	2.126
25	1.674	1.592	1.633	1.432
12.5	1.002	1.056	1.029	0.828
6.25	0.729	0.704	0.717	0.516
3.12	0.447	0.471	0.459	0.258
1.56	0.335	0.318	0.327	0.126
0.78	0.278	0.269	0.274	0.073
0	0.196	0.205	0.201

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Role of Pneumocystis jirovecii infection in chronic obstructive pulmonary disease progression in an immunosuppressed rat Pneumocystis pneumonia model T Xue,Experimental and Therapeutic Medicine,2020

产品评价

靶点详情

功能：

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
亚细胞定位：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle.
蛋白家族：

Small heat shock protein (HSP20) family
组织特异性：

Expressed in a variety of tissues. High levels in lung, adrenal, xiphoid, adipose tissue, heart and striated and smooth muscle, lower levels in the CNS. Adult levels are much higher in the slow-twitch soleus muscle than in the fast-twitch rectus femoris a
数据库链接：

STRING: 10116.ENSRNOP00000032902

UniGene: Rn.3841

Rat heat shock protein 27,HSP-27 ELISA Kit

产品详情

引用文献

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息