Rat heart fatty acid binding protein(h-FABP)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠心型脂肪酸结合蛋白(h-FABP)酶联免疫试剂盒（CSB-E16184r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FABP3含量。FABP3即脂肪酸结合蛋白3，在心肌、骨骼肌等组织中高表达。它可结合并转运长链脂肪酸，参与细胞内的脂质代谢。目前在心血管疾病等方面是研究热点，其机制研究有助于深入了解疾病发生发展，也为相关疾病诊断和治疗提供新方向。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，基础科研领域，如心肌损伤动物模型评估、心血管疾病病理机制研究、药物干预效果分析等场景，可兼容离体培养细胞分泌蛋白检测及心肌组织匀浆样本分析本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Fabp3Fatty acid-binding protein ELISA kit; heart ELISA kit; Fatty acid-binding protein 3 ELISA kit; Heart-type fatty acid-binding protein ELISA kit; H-FABP ELISA kit
缩写：

FABP3
Uniprot No.：

P07483
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat h-FABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	84-92
1:2	Average %	96
	Range %	91-102
1:4	Average %	84
	Range %	81-88
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of rat h-FABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-95
EDTA plasma (n=4)	103	95-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters.
基因功能参考文献：
1. Significant down-regulation of heart-type fatty acid binding protein after myocardial ischemia might play an important role in myocardial injury and energy metabolism disorder. PMID: 21155221
2. H-FABP might have potential to be an early and quantitative parameter of clinical severity and prognosis in CO poisoning. PMID: 20375122
3. A complex interrelationship between glucose and fatty acids in the control of FABP gene expression; FABP3 and 5 may play a role in insulin secretion. PMID: 21099311
4. rat myocardial FABP gene maps to chromosome 5q36 and was detected in a preparation of total RNA isolated from adult rat heart PMID: 10561574
亚细胞定位：

Cytoplasm.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
组织特异性：

Heart, but also skeletal muscle, kidney, brain and mammary gland.
数据库链接：

KEGG: rno:79131

STRING: 10116.ENSRNOP00000017325

UniGene: Rn.32566