Rat growth hormone releasing hormone,GHRH ELISA Kit
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中文名称：大鼠促生长激素释放激素(GHRH)酶联免疫试剂盒
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货号：CSB-E06878r
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规格：96T/48T
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价格：￥3900/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠促生长激素释放激素（GHRH）酶联免疫检测试剂盒（货号：CSB-E06878r），基于双抗体夹心法原理实现定量分析，适用于检测血清、血浆及组织匀浆样本中GHRH的表达水平。GHRH是由下丘脑分泌的关键调节肽，通过刺激垂体前叶释放生长激素（GH）参与机体生长调控、代谢平衡及组织修复等生理过程。该试剂盒检测灵敏度高，线性范围为3.12 pg/mL至200 pg/mL，可精准捕捉样本中微量GHRH的动态变化。实验操作采用标准化的预包被板与生物素化检测抗体体系，搭配显色底物完成信号检测，具有高特异性和重复性。本产品广泛应用于神经内分泌研究、生长激素相关疾病模型构建、药物干预效果评价等科研领域，为探索GHRH在生长发育、代谢疾病或应激反应中的调控机制提供可靠工具。试剂盒包含完整实验组分，支持常规实验室操作流程，适用于大鼠模型的基础研究与转化医学探索。
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别名：Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
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缩写：GHRH
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Uniprot No.：
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：3.12 pg/mL-200 pg/mL
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灵敏度：0.78 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Signal Transduction
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 97 Range % 92-103 1:2 Average % 92 Range % 85-99 1:4 Average % 90 Range % 88-94 1:8 Average % 101 Range % 96-105 -
回收率：
The recovery of rat GHRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 87-95 EDTA plasma (n=4) 106 103-111 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 200 2.088 2.252 2.170 2.080 100 1.585 1.676 1.631 1.541 50 1.163 1.104 1.134 1.044 25 0.831 0.765 0.798 0.708 12.5 0.550 0.524 0.537 0.447 6.25 0.344 0.345 0.345 0.255 3.12 0.182 0.183 0.183 0.093 0 0.090 0.089 0.090 -
数据处理：
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货期：3-5 working days
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靶点详情
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功能：GRF is released by the hypothalamus and acts on the adenohypophyse to stimulate the secretion of growth hormone.
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基因功能参考文献：
- GHRH agonistic analogs, JI-34 and MR-356 alleviated radiation-induced damage of cardiac myocytes. PMID: 27480202
- findings support the hypothesis that antagonizing the signaling of GHRH in T1D may improve GLP-1 function in the small intestine, which, in turn, diminishes TRL and reduces renal and vascular complications PMID: 26831066
- JI-38 had shown promising results in multiple fields including cardiology, diabetes and wound healing, our new, more potent, GHRH agonists should manifest additional efficacy for possible medical applications. PMID: 24373935
- GHRH protects the heart from ischemia/reperfusion injury through receptor-mediated mechanisms, leading to activation of RISK and SAFE pathways, which converge on mitochondria and possibly on AMP-activated protein kinase. PMID: 23417421
- We present evidence that growth retardation in upper airway obstruction is associated with a reduction in hypothalamic GHRH content. PMID: 21406516
- Growth hormone expression rises in pituitary gland during normal pregnancy, while its receptor mRNA level in hypothalamus decreases. PMID: 20814072
- GABAergic neurons in the median preoptic nucleus and ventrolateral preoptic nucleus are potential targets of the sleep-regulatory actions of GHRH. PMID: 19889861
- binding of [125I-Tyr(10)]hGHRH NH(2) to medullary homogenates was specific, time-dependent, reversible and saturable (K(d): 28 nM; B(max): 30 fmol/mg prot.). PMID: 11814616
- direct interactions between the growth hormone releasing hormone and growth hormone secretagogue receptors may explain the observed effects of of ghrelin and growth hormone secretagogues on signal transduction PMID: 12446584
- Review. Growth hormone-releasing hormone (GHRH) is a hypothalamic hormone that is essential for normal expansion of the somatotrope lineage during pituitary development. PMID: 12511850
- Changes in ghrelin and ghrelin receptor expression according to feeding status. PMID: 12876464
- Growth hormone releasing hormone (GHRH) is involved in the transcriptional regulation of the GH gene. PMID: 14664705
- results suggest that growth hormone secretagogue receptor is involved in the up-regulation of growth hormone releasing hormone and neuropeptide Y expression PMID: 16728494
- RFRP-3 infusion elicited a significant increase in growth hormone-releasing hormone mRNA and plasma growth hormone levels compared to control rats. PMID: 18628614
- Growth hormone-releasing hormone promotes survival of cardiac myocytes in vitro and protects against ischaemia-reperfusion injury in rat heart. PMID: 19293247
- Postsynaptic localization of sst(1) receptors and synaptic connectivity may be an important mechanism by which sst(1) mediates SRIF's cyclical effects on ARC GHRH neurons to generate the ultradian rhythm of GH secretion. PMID: 19553459
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亚细胞定位：Secreted.
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蛋白家族：Glucagon family
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数据库链接：
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