Rat growth differentiation factor 15(GDF15)ELISA Kit

Rat growth differentiation factor 15(GDF15)ELISA Kit

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中文名称：

大鼠生长分化因子15(GDF15)酶联免疫试剂盒
货号：

CSB-E16317r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO定量检测大鼠血清、血浆及组织匀浆中生长分化因子15（GDF15）的竞争法ELISA试剂盒，适用于科研领域研究该靶点的表达水平变化。GDF15属于TGF-β超家族成员，在调节炎症反应、能量代谢平衡及细胞应激反应中发挥重要作用，其异常表达与代谢性疾病、心血管病变及肿瘤微环境调控密切相关。试剂盒采用双抗体夹心竞争原理，样本中的GDF15与预包被抗体特异性结合后，通过酶标抗体显色反应实现定量检测，检测灵敏度达1.56 pg/mL，线性范围覆盖1.56-100 pg/mL，可精准识别低浓度样本差异。该试剂盒适用于能量代谢调控机制研究、心血管疾病动物模型评估、肿瘤相关病理分析等科研场景，配套标准品及预包被96孔板可实现高效批量检测，操作全程可在4小时内完成。实验需自备酶标仪、移液器等基础设备，建议初次实验时通过标准曲线建立优化样本稀释比例。
别名：

Gdf15 ELISA Kit; SbfGrowth/differentiation factor 15 ELISA Kit; GDF-15 ELISA Kit
缩写：

GDF15
Uniprot No.：

Q9Z0J6
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GDF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:10	Average %	92
	Range %	87-96
1:20	Average %	83
	Range %	80-86
1:40	Average %	96
	Range %	93-99
1:80	Average %	102
	Range %	99-105

回收率：

The recovery of rat GDF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-90
EDTA plasma (n=4)	93	89-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
100	0.127	0.129	0.128
50	0.208	0.220	0.214
25	0.334	0.324	0.329
12.5	0.472	0.498	0.485
6.25	0.713	0.735	0.724
3.12	1.162	1.139	1.151
1.56	1.542	1.567	1.555
0	2.814	2.864	2.839

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Liujunzi Decoction ameliorated cisplatin-induced anorexia by inhibiting the JAK-STAT signaling pathway and coordinating anorexigenic and orexigenic neuropeptides in rats Y Dai,Journal of ethnopharmacology,2023

功能：

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (Probable). On hepatocytes, inhibits growth hormone signaling.
基因功能参考文献：
1. These findings demonstrate the importance of the AP in the mediation of cancer-dependent anorexia and body weight loss and support a pathological role of MIC-1 as a tumour-derived factor mediating CACS, possibly via an AP-dependent action. PMID: 28025863
2. Plasma GDF-15 was increased 3, 12 and 26, while plasma TGF-beta was increased 12 weeks after irradiation. PMID: 27566082
3. the time course of the expression of growth differentiation factor15 (GDF15) in rat ischemic myocardium with increasing durations of reperfusion, and to elucidate its physiopathological role in the noreflow phenomenon, is reported. PMID: 26647773
4. findings indicate that the attenuation of REV-ERBalpha leads to an upregulation of Gdf10 and Gdf15 in decidual cells PMID: 26811051
5. GDF15 protects the renal interstitium and tubular compartment in experimental type 1 and 2 diabetes without affecting glomerular damage PMID: 23986522
6. Up-regulation of EGR-1 and NAG-1, along with higher reactive oxygen species load may positively regulate the intrinsic pathway of apoptosis for the NSAID mediated chemoprevention of colorectal cancer. PMID: 23435960
7. Gdf15 expression exhibits increases >10-fold at night in the rat pineal gland, reaching levels that are >15-fold higher than the median in other tissues. PMID: 19103603
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亚细胞定位：

Secreted.
蛋白家族：

TGF-beta family
组织特异性：

Detected in plasma (at protein level).
数据库链接：

KEGG: rno:29455

STRING: 10116.ENSRNOP00000026652

UniGene: Rn.44228

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.