Rat glycosaminoglycan/mucopolysaccharide ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专为大鼠样本设计的黏多糖/糖胺聚糖（GAGs）定量检测试剂盒，适用于血清、血浆及尿液样本的科研分析。黏多糖作为细胞外基质和结缔组织的重要成分，其代谢水平与多种生理调控及病理状态密切相关，在代谢性疾病模型研究、药物干预效果评估及组织修复机制探索中具有重要应用价值。试剂盒采用竞争法酶联免疫原理，通过标准品与样本中目标物对特异性抗体的竞争性结合实现精准检测，在6.25-100 ng/mL线性范围内实现定量分析，最低检测限达1.56 ng/mL。预包被板、标准品及检测抗体等核心组分均经过严格验证，支持96孔板高通量操作，全程实验仅需4.5小时即可完成。该试剂盒可广泛应用于大鼠疾病模型（如黏多糖贮积症、关节炎等）的病理机制研究、代谢调控实验及生物标志物筛选等科研领域，为研究者提供稳定可靠的糖胺聚糖定量检测方案。
缩写：

GAGs
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, urine
检测范围：

6.25 ng/mL-100 ng/mL
灵敏度：

3.125 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat glycosaminoglycan in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-97
1:200	Average %	98
	Range %	93-104
1:400	Average %	85
	Range %	81-88
1:800	Average %	88
	Range %	83-94

回收率：

The recovery of rat glycosaminoglycan spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.