Rat glucagon-like peptide-2,GLP-2 ELISA Kit

产品详情

产品描述：

CUSABIO双抗体夹心法定量检测大鼠血清、血浆及组织匀浆样本中胰高血糖素样肽2（Glp-2）的专用试剂盒，检测灵敏度达15.6 pg/mL，线性范围为15.6-1000 pg/mL（等同于15.6-1000 ng/L）。Glp-2作为肠道调节肽家族成员，在促进肠黏膜生长、营养吸收及肠道损伤修复等生理过程中发挥重要作用，是研究肠道功能与代谢调控的关键靶点。试剂盒采用高特异性配对抗体，通过预包被捕获抗体与样本中的Glp-2结合后，经酶标检测抗体显色反应实现精准定量分析，适用于营养学、消化系统疾病模型研究及药物干预实验中的Glp-2动态监测。该试剂盒可兼容多种生物基质样本，操作流程标准化，在4小时内即可完成检测，为研究肠道屏障功能、代谢综合征及肠上皮细胞增殖机制提供可靠的实验工具，满足体外科研实验中低丰度生物标志物的定量需求。
别名：

N/A
缩写：

Glp2
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat GLP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-95
1:2	Average %	98
	Range %	93-103
1:4	Average %	97
	Range %	90-104
1:8	Average %	86
	Range %	82-91

回收率：

The recovery of rat GLP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-99
EDTA plasma (n=4)	99	96-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.