Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

Rat fatty acid binding protein 4, adipocyte (FABP4) ELISA kit

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中文名称：

大鼠脂肪酸结合蛋白4，脂肪细胞型(FABP4)酶联免疫试剂盒
货号：

CSB-E13027r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO双抗体夹心法定量检测大鼠脂肪酸结合蛋白4（FABP4）的酶联免疫试剂盒，适用于血清、血浆、组织匀浆及细胞裂解液等多种样本的科研分析。FABP4是脂肪细胞特异性表达的脂质伴侣蛋白，参与脂肪酸运输与代谢调控，在肥胖、胰岛素抵抗及代谢综合征等病理过程中具有重要研究价值。试剂盒检测灵敏度达1.56 ng/mL，线性范围为1.56-100 ng/mL，采用高特异性抗体对实现精准定量，可满足细胞能量代谢研究、脂肪组织功能评估以及代谢性疾病机制探索等科研需求，例如在糖尿病模型大鼠中动态监测脂肪因子表达水平，或体外药物干预实验中评估化合物对脂肪细胞代谢的影响。试剂盒提供标准化操作流程，支持科研人员在体外实验模型中系统研究FABP4的生物学功能及其在代谢调节网络中的作用机制。
别名：

Fabp4Fatty acid-binding protein ELISA Kit; adipocyte ELISA Kit; Adipocyte lipid-binding protein ELISA Kit; ALBP ELISA Kit; Adipocyte-type fatty acid-binding protein ELISA Kit; A-FABP ELISA Kit; AFABP ELISA Kit; Fatty acid-binding protein 4 ELISA Kit
缩写：

FABP4
Uniprot No.：

P70623
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

1.56 ng/mL-100 ng/mL
灵敏度：

0.39 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat FABP4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-100
1:2	Average %	85
	Range %	80-90
1:4	Average %	89
	Range %	84-99
1:8	Average %	92
	Range %	86-101

回收率：

The recovery of rat FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	90	82-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	1.974	1.913	1.944	1.817
50	1.721	1.681	1.701	1.574
25	1.416	1.311	1.364	1.237
12.5	0.958	0.897	0.928	0.801
6.25	0.669	0.629	0.649	0.522
3.12	0.395	0.364	0.380	0.253
1.56	0.217	0.207	0.212	0.085
0	0.131	0.123	0.127

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

In utero exposure to the endocrine disruptor di-(2-ethylhexyl) phthalate induces long-term changes in gene expression in the adult male adrenal gland Martinez-Arguelles D et al,Endocrinology,2014

功能：

Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
亚细胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Calycin superfamily, Fatty-acid binding protein (FABP) family
数据库链接：

STRING: 10116.ENSRNOP00000014701

UniGene: Rn.4258

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.