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  4. Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

Rat extracellular superoxide dismutase [Cu-Zn](Cu/Zn-SOD/SOD3)ELISA kit

文献引用 (17) 说明书下载
  • 中文名称：
    大鼠胞外铜锌超氧化物歧化酶(Cu/Zn-SOD/SOD3)酶联免疫试剂盒
  • 货号：
    CSB-E14981r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO大鼠胞外铜锌超氧化物歧化酶（Cu/Zn-SOD/SOD3）酶联免疫检测试剂盒（货号：CSB-E14981r），专为科研领域设计，可定量检测血清、血浆、细胞培养上清液及组织匀浆样本中的SOD3蛋白水平。SOD3作为重要的抗氧化酶，通过催化超氧阴离子转化为过氧化氢和氧气，在胞外环境中发挥关键的氧化应激防御作用，其表达水平与多种生理病理过程密切相关。该试剂盒采用双抗体夹心法检测原理，通过特异性捕获抗体和检测抗体实现对目标蛋白的高灵敏度定量分析，检测范围覆盖15.6 pg/mL至1000 pg/mL，适用于研究氧化应激相关疾病模型、药物干预对SOD3活性的影响，以及细胞或组织微环境中抗氧化机制的动态变化。试剂盒操作流程标准化，兼容常规实验室设备，可满足神经退行性疾病、心血管疾病、炎症反应等研究领域中对SOD3蛋白表达水平的定量分析需求，为探索氧化还原平衡调控机制提供可靠工具。
  • 别名：
    Sod3 ELISA Kit; Sod-3 ELISA Kit; Extracellular superoxide dismutase [Cu-Zn] ELISA Kit; EC-SOD ELISA Kit; EC 1.15.1.1 ELISA Kit; Superoxide dismutase B ELISA Kit
  • 缩写：
    SOD3
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat Cu/Zn-SOD/SOD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:200 Average % 93  
    Range % 89-95  
    1:400 Average % 104  
    Range % 100-107  
    1:800 Average % 95  
    Range % 90-97  
    1:1600 Average % 98  
    Range % 91-101  
  • 回收率：
    The recovery of rat Cu/Zn-SOD/SOD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 95 92-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected  
    1000 2.644 2.588 2.616 2.461  
    500 2.264 2.344 2.304 2.149  
    250 1.650 1.632 1.641 1.486  
    125 1.044 1.028 1.036 0.881  
    62.5 0.624 0.636 0.630 0.475  
    31.2 0.383 0.368 0.376 0.221  
    15.6 0.248 0.239 0.244 0.089  
    0 0.156 0.154 0.155 　  
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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靶点详情

  • 功能：
    Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
  • 基因功能参考文献：
    1. Data show that aging is associated with reduction in superoxide dismutase (SOD) Cu/Zn-SOD protein expression and total SOD enzymatic activity in mesenteric lymphatic vessel (MLV). PMID: 22540739
    2. The impact of SOD3 on Akt and Erk1/2 cell survival pathways in rat hind limb injury model, is reported. PMID: 21909393
    3. ER stress leads to the down-regulation of claudin-5 among tight junction proteins and may induce the elevation of endothelial permeability and leakage of EC-SOD into the vitreous body. PMID: 21736484
    4. These data are important for understanding the pathogenesis of vascular diseases in which the synthesis of endothelial NO is decreased or extracellular O2- production exceeds the functional capacity of SOD3. PMID: 20033309
    5. Furin proteolytically processes the heparin-binding region of extracellular superoxide dismutase PMID: 11861638
    6. Levels are not significantly altered in the rat prostate during aging and thus is unlikely to be an important factor in the evolution of epithelial cell hyperplasia PMID: 16372329
    7. EcSOD, CuZnSOD, catalase, and MMP-2 mRNA expression did not statistically vary between aortic aneurysm and normal tissue PMID: 17196988
    8. SOD3 minimizes O2- levels preserving NO availability at resting conditions. SOD3 promotes NO mediated vasodilatation by scavenging O2- and basal SOD3 levels is able to inactivate O2- produced by 100% oxygen breathing preserving vasodilator effect of NO. PMID: 19198181
    9. SOD3 has a selective anti-inflammatory role in ischemic damages preventing the migration of reactive oxygen producing monocyte/macrophages PMID: 19495415

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  • 亚细胞定位：
    Secreted, extracellular space. Golgi apparatus, trans-Golgi network.
  • 蛋白家族：
    Cu-Zn superoxide dismutase family
  • 数据库链接：

    KEGG: rno:25352

    STRING: 10116.ENSRNOP00000005155

    UniGene: Rn.10358

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