Rat eosinophil cationic protein,ECP ELISA Kit

中文名称：

大鼠嗜酸性粒细胞阳离子蛋白(ECP)酶联免疫试剂盒
货号：

CSB-E11238r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO基于双抗体夹心原理开发的大鼠嗜酸性粒细胞阳离子蛋白（ECP）定量检测试剂盒（货号CSB-E11238r），适用于血清、血浆及组织匀浆样本的科研分析。ECP作为嗜酸性粒细胞释放的特征性毒性颗粒蛋白，在过敏性炎症、哮喘等疾病模型中具有重要研究价值，其水平变化常被用于评估嗜酸性粒细胞活化程度及相关病理过程。该试剂盒采用预包被特异性抗体的酶标板，通过双抗体夹心法实现62.5-4000 pg/mL的宽泛检测范围，最低检测限达25 pg/mL，可在4小时内完成实验流程。配套提供标准品、检测抗体及显色系统，适用于大鼠模型中的炎症机制研究、药效评估或疾病生物标志物探索，例如呼吸道炎症模型的病理进程监测、抗过敏药物干预效果分析等场景。实验全程无需特殊设备，支持常规酶标仪在450nm波长下进行读数，为科研工作者提供稳定可靠的ECP定量检测方案。
别名：

Rnase3 ELISA Kit; Ear11 ELISA Kit; Rns3 ELISA Kit; Eosinophil cationic protein ELISA Kit; ECP ELISA Kit; EC 3.1.27.- ELISA Kit; Eosinophil secondary granule ribonuclease 11 ELISA Kit; EAR-11 ELISA Kit; Ribonuclease 3 ELISA Kit; RNase 3 ELISA Kit
缩写：

RNASE3
Uniprot No.：

P70709
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	101
	Range %	96-107
1:4	Average %	92
	Range %	88-96
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	87	82-91

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.651	2.529	2.590	2.382
2000	2.004	1.903	1.954	1.746
1000	1.310	1.385	1.348	1.140
500	0.829	0.851	0.840	0.632
250	0.492	0.476	0.484	0.276
125	0.347	0.337	0.342	0.134
62.5	0.285	0.294	0.290	0.082
0	0.203	0.212	0.208

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Pulmonary toxicity of instilled silver nanoparticles: influence of size, coating and rat strain. Seiffert J et al,PLoS One,2015

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功能：

Cytotoxin and helminthotoxin with ribonuclease activity. Possesses a wide variety of biological activities.
亚细胞定位：

Cytoplasmic granule.
蛋白家族：

Pancreatic ribonuclease family
数据库链接：

KEGG: rno:100361866

STRING: 10116.ENSRNOP00000066375

UniGene: Rn.10626

Rat eosinophil cationic protein,ECP ELISA Kit

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