Rat diamine oxidase(DAO) ELISA Kit

Rat diamine oxidase(DAO) ELISA Kit

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中文名称：

大鼠二胺氧化酶(DAO)酶联免疫试剂盒
货号：

CSB-E12634r
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠二胺氧化酶(DAO)酶联免疫试剂盒（CSB-E12634r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DAO含量。DAO即去中心化自治组织，背景是随着区块链技术发展应运而生，旨在以智能合约实现组织的自治管理。其研究机制围绕如何通过代码和规则确保组织运作，减少人为干预，实现公平、透明决策，在金融、社区治理等领域有广泛应用前景。试剂盒检测范围为0.78 mIU/mL-50 mIU/mL，适用于肠道疾病模型研究、肠屏障功能评估、炎症机制探索等科研领域，例如通过检测实验动物血清或组织样本中DAO水平，研究肠道损伤修复、药物干预效果或病理生理过程。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Dao ELISA Kit; Dao1D-amino-acid oxidase ELISA Kit; DAAO ELISA Kit; DAMOX ELISA Kit; DAO ELISA Kit; EC 1.4.3.3 ELISA Kit
缩写：

DAO
Uniprot No.：

O35078
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.78 mIU/mL-50 mIU/mL
灵敏度：

0.195 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat DAO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	85
	Range %	80-91
1:200	Average %	93
	Range %	89-99
1:400	Average %	87
	Range %	82-95
1:800	Average %	101
	Range %	98-104

回收率：

The recovery of rat DAO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-92
EDTA plasma (n=4)	90	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml.	OD1	OD2	Average	Corrected
50	2.634	2.672	2.653	2.459
25	2.267	2.298	2.283	2.089
12.5	1.706	1.765	1.736	1.542
6.25	1.114	1.156	1.135	0.941
3.12	0.623	0.672	0.648	0.454
1.56	0.492	0.480	0.486	0.292
0.78	0.307	0.322	0.315	0.121
0	0.193	0.195	0.194

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Effects of Bile Acid Receptor TGR5 Agonist INT-777 on Intestinal Microflora Abundance and Metabolites in Rats with Acute Pancreatitis Z Zhang,/,/
Psyllium seed husk regulates the gut microbiota and improves mucosal barrier injury in the colon to attenuate renal injury in 5/6 nephrectomy rats D Hu,Renal failure,2023
Effect of TRAF6 in acute pancreatitis-induced intestinal barrier injury via TLR4/NF-κB signal pathway B Wei,Tissue & cell,2022
Effect of Electroacupuncture at Zusanli (ST36) on Sepsis Induced by Cecal Ligation Puncture and Its Relevance to Spleen D Xie,Evidence-Based Complementary and Alternative Medicine,2020
Cold-inducible RNA-binding protein maintains intestinal barrier during deep hypothermic circulatory arrest Li Y, et al,interactive Cardiovascular and Thoracic Surgery,2019
A combination of plasma DAO and citrulline levels as a potential marker for acute mesenteric ischemia Cakmaz R et al,Libyan J Med,2013

功能：

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
基因功能参考文献：
1. Plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment. PMID: 24005798
2. Its toxic effect on motor neurons can be mediated by expression in motor neurons and it promotes autophagy. PMID: 24138986
3. The study showed that the operation of renal ischemia resulted in a 45.49 +/- 8.30% (n = 8) decrease in the DAAO-induced consumption of substrate, indicating a sharp decrease in renal DAAO activity following this acute renal injury. PMID: 21110210
4. Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
5. results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198
6. This result clearly revealed that DAO was indispensable for the process of chiral inversion of d-leucine. PMID: 15026304
7. These results suggest that the TAA repeats are important for expression of the D-amino-acid oxidase gene. PMID: 18716858
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亚细胞定位：

Peroxisome.
蛋白家族：

DAMOX/DASOX family
数据库链接：

KEGG: rno:114027

STRING: 10116.ENSRNOP00000000881

UniGene: Rn.81058

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.