Rat cytochrome C oxidase(COX) ELISA Kit

产品详情

产品描述：

大鼠细胞色素C氧化酶(COX)酶联免疫试剂盒（CSB-E14281r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的COX含量。COX即环氧合酶，是催化花生四烯酸转化为前列腺素的限速酶。它有COX-1和COX-2两种同工酶，COX-1为结构型，参与维持机体生理功能；COX-2为诱导型，在炎症等病理状态下表达增加。其研究机制多围绕对这两种同工酶的作用开展。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于基础科研领域，如探究心血管疾病、神经退行性疾病或代谢综合征等模型中COX的活性变化，也可用于评估药物或环境因素对线粒体能量代谢通路的影响，为细胞呼吸机制研究、病理模型构建及药理实验提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

N/A
缩写：

COX
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat COX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	82-99
1:2	Average %	94
	Range %	86-100
1:4	Average %	93
	Range %	85-100
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of rat COX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-100
EDTA plasma (n=4)	96	89-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.