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  4. Rat cluster of differentiation 4,CD4 ELISA Kit

Rat cluster of differentiation 4,CD4 ELISA Kit

文献引用 (3) 说明书下载
  • 中文名称：
    大鼠CD4分子(CD4)酶联免疫试剂盒
  • 货号：
    CSB-E12928r
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专用于科研领域的大鼠CD4分子（CD4）酶联免疫检测试剂盒（货号：CSB-E12928r），基于双抗体夹心法原理实现定量分析，可精准检测大鼠血清、血浆及组织匀浆样本中CD4蛋白的表达水平。CD4作为T淋巴细胞表面重要的共受体分子，在抗原识别和免疫应答调控中发挥关键作用，其表达水平常被用于评估免疫系统功能状态及炎症相关疾病研究。该试剂盒检测灵敏度高，线性范围覆盖0.78 U/mL至50 U/mL，操作流程约需4.5小时，包含预包被特异性抗体的96孔板、标准品、检测抗体及显色底物等完整组分，所有组分均经过严格验证，确保批次间稳定性。试剂盒适用于免疫学基础研究、自身免疫性疾病模型评估、感染性疾病机制探索等方向，可为实验动物模型中T细胞功能分析、免疫调节药物开发等研究提供可靠数据支持。开封后试剂建议4℃保存并在1周内使用，未开封组分可于-20℃长期保存，避免反复冻融。
  • 别名：
    Cd4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; W3/25 antigen; CD antigen CD4
  • 缩写：
    CD4
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.78 U/mL-50 U/mL
  • 灵敏度：
    0.195 U/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat CD4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 97    
    Range % 87-102    
    1:2 Average % 101    
    Range % 94-105    
    1:4 Average % 99    
    Range % 88-103    
    1:8 Average % 105    
    Range % 98-109    
  • 回收率：
    The recovery of rat CD4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 92 85-98    
    EDTA plasma (n=4) 98 89-104    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    U/ml OD1 OD2 Average Corrected    
    50 2.656 2.587 2.622 2.508    
    25 2.171 2.062 2.117 2.003    
    12.5 1.438 1.388 1.413 1.299    
    6.25 0.720 0.715 0.718 0.604    
    3.12 0.412 0.424 0.418 0.304    
    1.56 0.261 0.252 0.257 0.143    
    0.78 0.176 0.183 0.180 0.066    
    0 0.112 0.115 0.114 　    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Cd4 Proteins

靶点详情

  • 功能：
    Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.
  • 基因功能参考文献：
    1. Adoptive transfusion of CD4+CD25+ Regulatory T Cells into Experimental Autoimmune Neuritis model rats has significant therapeutic effects. PMID: 29772575
    2. Increased C4d deposition may be amenable to identify early myocardial infarction after cardiac arrest. PMID: 26467126
    3. Data show several homologies across human, rat and mouse in the kinetic of loss of several LAgs such as CD5, CD4 and CD28. PMID: 21745657
    4. testicular macrophages expressed the markers CD45 and MAC387 and most also expressed CD4. PMID: 11994538
    5. C4d deposition and cellular infiltrates as markers of acute rejection in rat models of orthotopic lung transplantation. PMID: 18622289
    6. In this EAE model, perivascular cells, microglia and macrophages showed different dynamics; microglial cells expressed CD4 during the recovery phase, suggesting microglial CD4 expression in the resolution of the immune response. PMID: 19246105
    7. Clear trend toward worse graft survival in patients with focal C4d after kidney transplantaation. PMID: 19344468
    8. Capillary C4d deposition is a marker of antibody-mediated rejection in kidney transplantation and can be detected by various staining methods. PMID: 19376373

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 数据库链接：

    KEGG: rno:24932

    STRING: 10116.ENSRNOP00000021915

    UniGene: Rn.10748

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |