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Rat calcitonin gene related peptide,CGRP ELISA Kit

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CUSABIO大鼠降钙素基因相关肽（CGRP）酶联免疫检测试剂盒（货号：CSB-E08211r），采用双抗体夹心法定量检测血清、血浆或组织匀浆样本中的CGRP水平，检测灵敏度为6.25 pg/mL，线性范围覆盖6.25至400 pg/mL。CGRP是一种由感觉神经末梢释放的神经肽，广泛参与神经源性炎症、血管舒张及痛觉调节等生理过程，其表达水平与偏头痛、心血管疾病及神经退行性疾病的研究密切相关。本试剂盒通过特异性抗体对实现靶标的高精度捕获与检测，适用于科研场景中CGRP的定量分析，例如神经生物学研究中的痛觉传导机制探索、心血管疾病模型中的血管舒缩功能评估，或药理学实验中对CGRP调控类化合物作用的体外验证。试剂盒操作流程标准化，提供预包被酶标板及完整检测组分，可在常规实验室条件下完成样本检测，为相关疾病机制研究及药物开发评估提供可靠数据支持。
别名：

Calcb ELISA Kit; Calcitonin gene-related peptide 2 ELISA Kit; Beta-type CGRP ELISA Kit; Calcitonin gene-related peptide II ELISA Kit; CGRP-II ELISA Kit
缩写：

CGRP
Uniprot No.：

P10093
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat CGRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	80-95
1:2	Average %	94
	Range %	87-101
1:4	Average %	90
	Range %	86-106
1:8	Average %	95
	Range %	82-100

回收率：

The recovery of rat CGRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	94	88-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.742	2.725	2.734	2.562
200	2.137	2.183	2.160	1.988
100	1.384	1.481	1.433	1.261
50	0.863	0.806	0.835	0.663
25	0.452	0.475	0.464	0.292
12.5	0.298	0.292	0.295	0.123
6.25	0.203	0.214	0.209	0.037
0	0.171	0.173	0.172

本试剂盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-Rat CGRP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled CGRP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本试剂盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.