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中文名称：大鼠β胶原交联(bCTx)酶联免疫试剂盒
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货号：CSB-EQ027521RA
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：大鼠β胶原交联(bCTx)酶联免疫试剂盒（CSB-EQ027521RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的bCTx含量。试剂盒检测范围为7.8 pg/mL-500 pg/mL，在骨质疏松机制研究、骨病模型评价及骨代谢相关药物研发中具有重要科研价值；广泛应用于骨代谢动态监测、抗骨吸收药物疗效评估、骨折愈合研究及骨肿瘤模型构建等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
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缩写：bCTx
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma, tissue homogenates
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检测范围：7.8 pg/mL-500 pg/mL
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灵敏度：1.95 pg/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Others
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat bCTx in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 88-96 1:2 Average % 96 Range % 93-99 1:4 Average % 102 Range % 98-106 1:8 Average % 87 Range % 84-90 -
回收率：
The recovery of rat bCTx spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 87-95 EDTA plasma (n=4) 101 97-106 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 500 2.793 2.878 2.836 2.634 250 1.953 1.830 1.892 1.690 125 1.153 1.215 1.184 0.982 62.5 0.745 0.761 0.753 0.551 31.2 0.532 0.545 0.539 0.337 15.6 0.436 0.405 0.421 0.219 7.8 0.314 0.301 0.308 0.106 0 0.205 0.199 0.202 -
数据处理：
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货期：3-5 working days
引用文献
- Metabolomics and physiological analysis of the effect of calcium supplements on reducing bone loss in ovariectomized rats by increasing estradiol levels H Mao,Nutrition & metabolism,2021
- The Impact of Strontium Ranelate on Metaphyseal Bone Healing in Ovariectomized Rats Komrakova M. et al,Calcif Tissue Int.,2015
- Neutrophil serine proteases and their endogenous inhibitors in coronary artery ectasia patients Ruifeng Liu et al,Anatol J Cardiol,2015
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