Rat apolipoprotein H (Apo-H) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠载脂蛋白H(Apo-H)酶联免疫试剂盒（CSB-E10073r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的APOH含量。APOH（载脂蛋白H）是一种参与脂质代谢的关键蛋白，在调节凝血和炎症方面发挥着重要作用。研究表明，APOH与多种疾病有关，包括自身免疫性疾病和心血管疾病。其研究机制主要涉及APOH与带负电物质（如肝素和磷脂）的相互作用，以及其在防止内在凝血级联激活中的作用。试剂盒检测范围为28 ng/mL-1800 ng/mL，适用于大鼠模型中Apo-H的定量分析，可支持脂代谢研究、心血管疾病机制探索以及免疫相关实验的开展，特别适用于基础科研中涉及脂蛋白调控、血栓形成或免疫应答机制的相关课题。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

ApohBeta-2-glycoprotein 1 ELISA kit; Apolipoprotein H ELISA kit; Apo-H ELISA kit; Beta-2-glycoprotein I ELISA kit; B2GPI ELISA kit; Beta(2)GPI ELISA kit
缩写：

APOH
Uniprot No.：

P26644
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

28 ng/mL-1800 ng/mL
灵敏度：

7 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-H in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	102
	Range %	97-107
1:800	Average %	91
	Range %	85-97
1:1600	Average %	97
	Range %	91-103

回收率：

The recovery of rat Apo-H spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	107	101-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.
亚细胞定位：

Secreted.
组织特异性：

Expressed by the liver and secreted in plasma.
数据库链接：

UniGene: Rn.1824