Rat apolipoprotein B-48 (ApoB48) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO专用于定量检测大鼠血清、血浆、细胞培养上清液及组织匀浆样本中载脂蛋白B-48（ApoB48）的酶联免疫试剂盒。ApoB48是乳糜微粒核心蛋白成分，参与肠道脂质吸收与转运，其表达水平与脂代谢异常及动脉粥样硬化等病理过程密切相关，是研究脂蛋白代谢机制的重要生物标志物。试剂盒基于竞争法原理设计，采用预包被特异性抗体的微孔板，样本中的ApoB48与HRP标记的检测抗原竞争结合抗体，通过显色反应强度（OD值）反比计算目标蛋白浓度，检测灵敏度为0.312 μg/mL，线性范围为0.312-20 μg/mL，可精确检测低丰度样本。该试剂盒适用于营养干预实验、代谢性疾病模型研究、药物对肠道脂质吸收影响的评估等科研领域，操作流程包含样本前处理、孵育、洗涤及比色步骤，配备标准品与实验所需全套试剂组分，实验全程约需3小时完成，为探索脂代谢调控机制及心血管疾病相关研究提供可靠工具。
缩写：

ApoB48
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.312 μg/mL-20 μg/mL
灵敏度：

0.078 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ApoB48 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	88
	Range %	84-92
1:200	Average %	96
	Range %	92-102
1:400	Average %	95
	Range %	89-100
1:800	Average %	92
	Range %	89-96

回收率：

The recovery of rat ApoB48 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	92	88-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.