Rat apolipoprotein A1 (Apo-A1) ELISA Kit

Rat apolipoprotein A1 (Apo-A1) ELISA Kit

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中文名称：

大鼠载脂蛋白A1(Apo-A1)酶联免疫试剂盒
货号：

CSB-E08105r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO专用于科研领域的大鼠载脂蛋白A1（Apo-A1）酶联免疫检测试剂盒（货号：CSB-E08105r），基于双抗体夹心法原理实现定量分析，可精准检测血清、血浆及组织匀浆样本中Apo-A1蛋白的表达水平，检测灵敏度达0.14 ng/ml，线性范围为0.14-100 ng/ml。Apo-A1是高密度脂蛋白（HDL）的核心结构蛋白，在胆固醇逆向转运和心血管保护中发挥关键作用，其表达水平与脂代谢调控、动脉粥样硬化等病理过程密切相关。本试剂盒适用于基础科研中对大鼠模型脂代谢机制的研究，例如通过检测不同实验条件下（如基因干预、饮食调控或药物处理）血清或组织中的Apo-A1浓度变化，评估心血管疾病模型的病理进程或干预效果。实验操作兼容常规实验室设备，提供标准化的检测流程，确保数据的可靠性和重复性，为探索Apo-A1在脂质稳态及代谢性疾病中的功能提供高效研究工具。
别名：

Apoa1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I)] ELISA kit
缩写：

APOA1
Uniprot No.：

P04639
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.14 ng/ml-100 ng/ml.
灵敏度：

0.14 ng/ml.
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat Apo-A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1000	Average %	94
	Range %	88-108
1:2000	Average %	97
	Range %	91-105
1:4000	Average %	103
	Range %	98-108
1:8000	Average %	93
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

100

2.340

2.434

2.387

2.275

33.33

1.892

1.867

1.880

1.768

11.11

1.157

1.128

1.142

1.031

3.70

0.625

0.639

0.632

0.521

1.23

0.297

0.311

0.304

0.192

0.41

0.188

0.193

0.190

0.079

0.14

0.134

0.140

0.137

0.025

0.108

0.115

0.112

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Hypolipidemic and anti-inflammatory effects of aorta and heart tissues of cattle and pigs in the atherosclerosis rat model Chernukha IM.et al,Anim Sci J,2018
Effect of n-3 PUFA supplementation at different EPA: DHA ratios on the spontaneously hypertensive obese rat model of the metabolic syndrome Molinar-Toribio E et al,Br J Nutr,2015

最新研究进展：

APOA1是一种高密度脂蛋白(HDL)的主要成分，参与调节胆固醇代谢和清除血管内的胆固醇。最新研究表明，APOA1可以抑制乳腺癌的恶性转化和肺癌细胞的增殖，可能成为肿瘤治疗的新靶点。此外，APOA1水平与糖尿病、心血管疾病和肝病等多种疾病相关。
功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能参考文献：
1. both the route of administration and the formulation of apoA-I peptide significantly affect its pharmacokinetics and pharmacodynamics. PMID: 27881716
2. ABCA1 is diminished and ABCG1 is increased by N-acetylcysteine, which counteracts the reduction in apo A-I-mediated cholesterol efflux PMID: 25305669
3. Apolipoprotein A-I induces tubulin phosphorylation in association with cholesterol release in fetal rat astrocytes. PMID: 24814947
4. ApoA-I may be a protective blood-borne factor involved in the remote ischemic preconditioning mechanism. PMID: 24155931
5. It maintains cholesterol homeostasis. PMID: 24117066
6. on neonatal rat ventricular cardiomyocytes, the aldosterone-dependent chronotropic effects of antiapoA-1 IgG involve the nongenomic activation of L-type calcium channels PMID: 22253414
7. translocation of cholesterol and phospholipid into the cytosol is related to the apo A-I-mediated HDL assembly in astrocytes through functional association with caveolin-1 and a cyclosporin A-sensitive cyclophilin protein(s). PMID: 11773045
8. ABCA1-dependent cholesterol mobilization to apoA-I increased new cholesterol synthesis, indicating depletion of the regulatory pool of hepatocyte cholesterol during HDL formation PMID: 14993246
9. The association of CLPP with microtubules is mediated by caveolin-1, induced by apoA-I and regulates intracellular cholesterol trafficking for assembly of cellular lipids to apoA-I-HDL. PMID: 16606364
10. Data show that a complex of apolipoprotein A-I and pregnenolone modulate the rate of protein biosynthesis in liver cells. PMID: 18457017
11. ApoA-I reduced I/R-induced inflammatory responses, decreased renal microscopic damage and improved renal function. ApoA-I protects kidney from I/R injury by inhibiting inflammatory cytokines release and neutrophil infiltration and activation. PMID: 18535924
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亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
组织特异性：

Major protein of plasma HDL, also found in chylomicrons.
数据库链接：

KEGG: rno:25081

STRING: 10116.ENSRNOP00000065206

UniGene: Rn.10308

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.