Rat anti-Thyroid-Peroxidase antibody,TPO-Ab ELISA Kit

产品详情

产品描述：

CUSABIO试剂盒采用竞争法原理，定量检测大鼠血清或血浆样本中的甲状腺过氧化物酶抗体（TPO-Ab），检测范围为5-80 IU/mL。TPO-Ab是针对甲状腺过氧化物酶的自身抗体，其异常升高与自身免疫性甲状腺疾病（如桥本甲状腺炎、Graves病）密切相关，是评估甲状腺自身免疫状态的重要指标。本产品通过高特异性抗体与样本中的TPO-Ab竞争结合包被抗原，结合酶标二抗显色反应实现精准定量，适用于科研场景下甲状腺疾病发病机制研究、药物干预效果评估或动物模型中的免疫状态分析。试剂盒严格验证检测灵敏度与线性范围，样本兼容血清及血浆（含EDTA、肝素、枸橼酸盐抗凝），可满足实验室对大鼠样本中TPO-Ab水平的定量检测需求，为甲状腺相关免疫学研究提供可靠工具。
缩写：

TPO Ab
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma
检测范围：

5 IU/mL-80 IU/mL
灵敏度：

5 IU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat anti-TPO antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	86-101
1:2	Average %	88
	Range %	83-98
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rat anti-TPO antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-99
EDTA plasma (n=4)	95	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

平均分:

4.5分 - 2 个评价

样品类型：血清

样品信息：大鼠

稀释比：其他正常组1:5、模型组1:10

产品评价：实验结果见下图。标准品可即用，能够节省时间；但是板子是12孔一排，拆卸有难度。总体满意。

By 谢老师

样品类型：血清

样品信息：大鼠

稀释比：其他 1：10

产品评价：我用CSB-E11199r检测自身免疫性甲状腺炎大鼠，稀释比例为1：10。自身免疫性甲状腺炎最重要的就是甲状腺抗体TPOAb和TGAb，我们实验室试了很多厂家，总结得出武汉华美的甲状腺的elisa试剂盒实验结果是最准的，好用性价比高！

By 丁老师