Rat alcohol dehydrogenase,ADH ELISA Kit

Rat alcohol dehydrogenase,ADH ELISA Kit

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中文名称：

大鼠乙醇脱氢酶(ADH)酶联免疫试剂盒
货号：

CSB-E11180r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠乙醇脱氢酶(ADH)酶联免疫试剂盒（CSB-E11180r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ADH含量。ADH即抗利尿激素，它主要由下丘脑视上核和室旁核的神经内分泌细胞合成。其作用机制主要是增加肾小管对水的重吸收，以维持机体水平衡。在临床上，ADH水平异常会引发如尿崩症等疾病，相关研究有助于深入了解水代谢调节机制与疾病治疗。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于体外研究乙醇代谢调控机制、评估肝损伤模型中ADH表达水平动态变化，或探究药物干预对酶活性影响的实验场景；为研究乙醇代谢通路、肝脏功能评估及毒性物质作用机制提供高效的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Adh1 ELISA Kit; Adh-1 ELISA Kit; Alcohol dehydrogenase 1 ELISA Kit; EC 1.1.1.1 ELISA Kit; Alcohol dehydrogenase A subunit ELISA Kit
缩写：

ADH
Uniprot No.：

P06757
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat ADH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	86-95
1:2	Average %	101
	Range %	96-105
1:4	Average %	93
	Range %	88-97
1:8	Average %	97
	Range %	91-103

回收率：

The recovery of rat ADH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-99
EDTA plasma (n=4)	88	80-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.312	2.456	2.384	2.237
10	1.586	1.712	1.649	1.502
5	1.009	1.054	1.032	0.885
2.5	0.641	0.632	0.637	0.490
1.25	0.412	0.435	0.424	0.277
0.625	0.287	0.274	0.281	0.134
0.312	0.211	0.218	0.215	0.068
0	0.146	0.147	0.147

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

基因功能参考文献：
1. study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism. PMID: 22214999
2. rat hepatic Class I ADH gene expression is regulated in vivo by ethanol in a mechanism involving the C/EBP site and the enhancer binding proteins beta and gamma PMID: 12213809
3. Results describe the expression patterns of alcohol dehydrogenases I, III, and IV in adult rat, mouse and human tissues using radioactive oligonucleotide in situ hybridization. PMID: 12631290
4. activity and expression in gastrointestinal tissue homogenates PMID: 12787032
5. class I alcohol dehydrogenase is induced by sterol regulatory element-binding protein-1 in rat liver PMID: 15123720
6. Alcohol dehydrogenase PMID: 16109828
7. Cellular expression of Adh1 in the GI tract was studied. PMID: 17257171
8. A high ADH activity leads to low ethanol intake in animals. PMID: 17488809
9. ADH class I gene is progesterone-responsive in the uterus. PMID: 18219182
10. Results revealed that kidney alcohol dehydrogenase activities increased significantly after ethanol administration, but the electrophoretic pattern of alcohol dehydrogenase isoforms was unmodified. PMID: 16421892
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亚细胞定位：

Cytoplasm.
蛋白家族：

Zinc-containing alcohol dehydrogenase family, Class-I subfamily
数据库链接：

KEGG: rno:24172

STRING: 10116.ENSRNOP00000016346

UniGene: Rn.40222

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.