Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

Rat Visceral adipose-specific serine protease inhibitor,vaspin ELISA Kit

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中文名称：

大鼠内脏脂肪特异性丝氨酸蛋白酶抑制剂(vaspin)酶联免疫试剂盒
货号：

CSB-E09813r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠内脏脂肪特异性丝氨酸蛋白酶抑制剂（vaspin）定量检测试剂盒（货号CSB-E09813r），采用双抗体夹心ELISA原理开发，可精准检测血清、血浆及组织匀浆样本中vaspin蛋白的表达水平。vaspin作为脂肪细胞分泌的重要脂肪因子，在调节胰岛素敏感性、糖代谢平衡及炎症反应中发挥关键作用，其水平变化与肥胖相关代谢综合征、2型糖尿病等病理机制密切相关。试剂盒检测灵敏度高，线性范围覆盖31.25至2000 pg/mL，适用于微量样本中目标蛋白的准确定量分析。实验操作基于预包被特异性捕获抗体的酶标板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，最终在450nm波长下通过酶标仪读取吸光度值进行定量。该试剂盒可广泛应用于肥胖动物模型建立、代谢性疾病机制研究、脂肪组织功能评估以及药物干预效果评价等科研领域，为探索vaspin在能量代谢调控中的分子机制提供可靠工具。实验需自备酶标仪、微量移液器等基础设备，所有组分经严格质控确保批次稳定性。
别名：

Serpina12 ELISA Kit; Serpin A12 ELISA Kit; Visceral adipose tissue-derived serine protease inhibitor ELISA Kit; Vaspin ELISA Kit; Visceral adipose-specific serpin ELISA Kit
缩写：

SERPINA12
Uniprot No.：

Q8R4Z1
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat vaspin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	94
	Range %	90-98
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	92-104

回收率：

The recovery of rat vaspin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	105	100-110
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.679	2.576	2.628	2.479
1000	2.071	2.114	2.093	1.944
500	1.582	1.472	1.527	1.378
250	0.958	0.913	0.936	0.787
125	0.580	0.589	0.585	0.436
62.5	0.412	0.407	0.410	0.261
31.25	0.302	0.279	0.291	0.142
0	0.147	0.151	0.149

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Adipokine that modulates insulin action by specifically inhibiting its target protease KLK7 in white adipose tissues.
亚细胞定位：

Secreted.
蛋白家族：

Serpin family
组织特异性：

Expressed in visceral adipose tissues.
数据库链接：

STRING: 10116.ENSRNOP00000012960

UniGene: Rn.79035

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.