Rat Vasoactive Intestinal Peptide,VIP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO大鼠血管活性肠肽（VIP）酶联免疫试剂盒（货号：CSB-E08355r），采用双抗体夹心法定量检测血清、血浆及组织匀浆样本中的VIP含量。血管活性肠肽是一种广泛分布于胃肠系统和神经系统的多肽类神经递质，参与调节肠道蠕动、血管舒张及免疫炎症反应等多种生理过程，其水平变化与胃肠功能紊乱、神经内分泌调控密切相关。本试剂盒检测范围为1.56-100 pg/mL，最低检测限达1.56 pg/mL，可精准捕捉微量VIP浓度变化。实验采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统显色，配合标准曲线实现目标物定量分析。适用于神经生物学、消化系统疾病模型研究，可分析大鼠外周循环或组织局部VIP表达水平，为探究胃肠动力障碍、炎症调节机制及神经-免疫交互作用提供数据支持。本产品严格限定于科研用途，操作时需按规范处理生物样本，避免反复冻融及蛋白酶干扰。
缩写：

VIP
Uniprot No.：

P32649 P01283
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

1.56 pg/mL-100 pg/mL
灵敏度：

0.39 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat VIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	97
	Range %	93-101
1:4	Average %	100
	Range %	97-103
1:8	Average %	86
	Range %	82-92

回收率：

The recovery of rat VIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	90	86-94

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.