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  4. Rat Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR ELISA kit

Rat Urokinase-type Plasminogen Activator Receptor,PLAUR/uPAR ELISA kit

说明书下载
  • 中文名称：
    大鼠尿激酶型纤溶酶原激活物受体(PLAUR/uPAR)酶联免疫试剂盒
  • 货号：
    CSB-E07366r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠尿激酶型纤溶酶原激活物受体(PLAUR/uPAR)酶联免疫试剂盒（CSB-E07366r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PLAUR含量。PLAUR即尿激酶型纤溶酶原激活物受体，在细胞表面参与调节纤溶、细胞迁移等过程。其背景上和多种生理病理活动相关，尤其是肿瘤进展。研究机制主要是激活纤溶酶原，促进细胞外基质降解，还能与多种信号通路相互作用，影响细胞增殖、侵袭等。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于探索PLAUR/uPAR在疾病模型中的表达调控机制、生物标志物筛选或药理学研究中的靶点验证。在肿瘤侵袭转移、炎症反应及纤维化疾病研究中具有重要价值。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    PlaurUrokinase plasminogen activator surface receptor ELISA kit; U-PAR ELISA kit; uPAR ELISA kit; CD antigen CD87 ELISA kit
  • 缩写：
    PLAUR
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    0.156 ng/mL-10 ng/mL
  • 灵敏度：
    0.039 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cancer
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PLAUR/uPAR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 84-92
    1:2 Average % 103
    Range % 98-106
    1:4 Average % 95
    Range % 88-99
    1:8 Average % 92
    Range % 87-96
  • 回收率：
    The recovery of rat PLAUR/uPAR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 92 83-96
    EDTA plasma (n=4) 104 96-108
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    10 2.113 2.273 2.193 2.072
    5 1.643 1.732 1.688 1.567
    2.5 1.072 1.119 1.096 0.975
    1.25 0.682 0.691 0.687 0.566
    0.625 0.404 0.418 0.411 0.290
    0.312 0.276 0.289 0.283 0.162
    0.156 0.206 0.203 0.205 0.084
    0 0.120 0.121 0.121 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

产品评价

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Plaur antibodies

Plaur Proteins

靶点详情

  • 功能：
    Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA.
  • 基因功能参考文献：
    1. Increased postmortem PLAUR expression could serve as a biomarker to aid diagnosis of early myocardial infarction. PMID: 24935436
    2. Rapamycin could promote podocyte migration through the up-regulation of uPAR leading to proteinuria. PMID: 24815166
    3. uPA, uPAR, MMP-2 and MMP-9 play an important role in GBM growth. Blockade of uPA and interruption of the proteolytic cascade could become a useful tool in the therapy of GBM PMID: 22773570
    4. uPAR associates with activating enhancer-binding protein 2alpha (AP2a) and mediates beta-catenin gene transcription. PMID: 22511755
    5. study examines the expression and function of uPAR in developing rat ventral prostates PMID: 11798065
    6. Expression pattern of the urokinase-plasminogen activator system in rat DS-sarcoma: role of oxygenation status and tumour size PMID: 11953898
    7. Decreased expression of intercellular adhesion molecule-1 (ICAM-1) is associated with tumor cell spreading in vivo. PMID: 12198772
    8. demonstrated focused activation of proteolytic enzymatic activity in several cell types and showed that the uPA/uPAR system is an important contributor to focal pericellular proteolysis PMID: 15042374
    9. Altered expression of uPAR in diabetic nephropathy was associated with mesangial expansion. PMID: 15322501
    10. Domain 2 of the urokinase receptor plays a pivotal role in the regulation of uPAR-integrin alphavbeta3 interactions PMID: 15863511
    11. The members of caspases, MEK1/2, PKC, and NF-kappaB are involved in TRAIL-induced expression of uPA, IL-8, MMP-7 and MMP-9. PMID: 18397859
    12. In animals undergoing SE, uPAR expression increased dramatically, peaking at 1 and 4 days after SE. Increased uPAR expression during post-injury phase supports its contribution to tissue remodeling in the brain. PMID: 19527776

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  • 亚细胞定位：
    [Isoform 1]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 2]: Secreted.
  • 数据库链接：

    KEGG: rno:50692

    STRING: 10116.ENSRNOP00000063960

    UniGene: Rn.82711

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |