Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

Rat Tripartite motif-containing protein 72(TRIM72) ELISA kit

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中文名称：

大鼠三结构域包含蛋白72(TRIM72)酶联免疫试剂盒
货号：

CSB-EL024511RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒用于定量检测大鼠血清、血浆、组织匀浆或细胞裂解液中的三结构域包含蛋白72（TRIM72），该蛋白属于TRIM家族成员，通过参与细胞膜修复和应激反应调控在组织损伤保护中发挥重要作用，是心血管疾病、肌肉再生等领域的研究热点。采用双抗体夹心法原理，在18.75-1200 pg/mL线性范围内实现精准定量，最低检测限为7.5 pg/mL，实验全程约需4.5小时，包含预包被捕获抗体、生物素化检测抗体及标准品等核心组分。适用于研究TRIM72在心肌缺血再灌注损伤模型中的动态表达、骨骼肌再生机制探索或药物干预后生物标志物水平变化的定量分析，实验时需注意样本采集后避免反复冻融，血浆推荐使用EDTA或肝素抗凝处理，组织样本建议按1:9比例稀释匀浆以获得最佳检测结果。本试剂盒提供96孔板规格，操作流程标准化且兼容常规酶标仪检测。
别名：

Trim72 ELISA Kit; Mg53 ELISA Kit; Tripartite motif-containing protein 72 ELISA Kit; Mitsugumin-53 ELISA Kit; Mg53 ELISA Kit
缩写：

TRIM72
Uniprot No.：

A0JPQ4
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

18.75 pg/mL-1200 pg/mL
灵敏度：

4.7 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TRIM72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	97
	Range %	91-105
1:4	Average %	99
	Range %	92-110
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rat TRIM72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-98
EDTA plasma (n=4)	96	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.814	2.989	2.902	2.817
600	2.366	2.441	2.404	2.319
300	1.559	1.457	1.508	1.423
150	0.753	0.697	0.725	0.640
75	0.469	0.442	0.456	0.371
37.5	0.243	0.235	0.239	0.154
18.75	0.149	0.139	0.144	0.059
0	0.088	0.082	0.085

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles.
基因功能参考文献：
1. These results suggest that an 8-week swimming exercise improves HFD-induced insulin resistance maybe through a reduction of TRIM72 in skeletal muscle and enhancement of AKT signal transduction. PMID: 27843952
2. MG53 is an effective biomarker of myocardial injury and dysfunction in murine hearts. However, MG53 is not expressed in human heart and therefore does not hold utility as a clinical biomarker of myocardial injury PMID: 26790476
3. MG53 is a facilitator of rapid injury repair, a mediator of cell migration, and a modulator of myofibroblast differentiation during wound healing PMID: 26306047
4. MG53, a member of the muscle-specific transmembrane adaptor molecule (TRIM) protein family, is a crucial component of the cardiac ischemic preconditioning response. PMID: 20516375
亚细胞定位：

Cell membrane, sarcolemma. Cytoplasmic vesicle membrane.
蛋白家族：

TRIM/RBCC family
数据库链接：

KEGG: rno:365377

STRING: 10116.ENSRNOP00000030976

UniGene: Rn.23565

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.