Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

Rat Tissue factor pathway inhibitor,TFPI ELISA Kit

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中文名称：

大鼠组织因子途径抑制物(TFPI)酶联免疫试剂盒
货号：

CSB-E08261r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠组织因子途径抑制物(TFPI)酶联免疫试剂盒（CSB-E08261r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的TFPI含量。TFPI即组织因子途径抑制物，是一种天然存在的抗凝蛋白。它能抑制组织因子（TF）介导的凝血途径，通过与因子Xa和TF - 因子VIIa复合物结合，发挥抗凝作用。其在调节凝血平衡中起关键作用，对其研究有助于开发新型抗凝药物等。试剂盒检测范围为7.8 ng/mL-500 ng/mL，为研究凝血机制、心血管疾病模型、药物干预实验及炎症相关研究提供可靠工具，特别适用于评估凝血相关因子调控、病理模型建立及治疗性化合物筛选等科研场景，所有检测结果仅用于科学研究目的。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Tfpi ELISA Kit; Tissue factor pathway inhibitor ELISA Kit; TFPI ELISA Kit; Extrinsic pathway inhibitor ELISA Kit; EPI ELISA Kit; Lipoprotein-associated coagulation inhibitor ELISA Kit; LACI ELISA Kit
缩写：

TFPI
Uniprot No.：

Q02445
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TFPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	85-96
1:2	Average %	89
	Range %	84-95
1:4	Average %	93
	Range %	89-97
1:8	Average %	90
	Range %	85-94

回收率：

The recovery of rat TFPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-107
EDTA plasma (n=4)	92	88-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
500	2.596	2.474	2.535	2.424
250	1.978	1.902	1.940	1.829
125	1.172	1.096	1.134	1.023
62.5	0.687	0.702	0.695	0.584
31.2	0.401	0.424	0.413	0.302
15.6	0.282	0.291	0.287	0.176
7.8	0.182	0.175	0.179	0.068
0	0.113	0.108	0.111

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Hemostatic effect of acylated ghrelin in control and sleeve gastrectomy-induced rats: mechanisms of action Morsy MD.et al,ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY,2018
Procoagulant and Fibrinolytic Activity after Polytrauma in Rat Wu X. et al,Am J Physiol Regul Integr Comp Physiol,2015

功能：

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.
基因功能参考文献：
1. TFPI expression is induced in cardiomyocytes, but not in endothelial cells, by IL-1beta. PMID: 11816723
2. Rapid atrial pacing acutely downregulated the gene expression of thrombomodulin and TFPI in rat atrial endocardium, thereby inducing local coagulation imbalance on the internal surface of the atrial cavity PMID: 14610015
3. TFPI levels decreased significantly at 24 hours in burn, septic injury, and burn+septic injury groups, followed by incomplete rebound recovery at 72 hours PMID: 15497025
4. This is the first report showing that direct inhibition of TF-mediated coagulation activation abrogates bleomycin-induced pulmonary fibrosis. PMID: 16338226
5. analysis of tissue factor changes and the effects of tissue factor pathway inhibitor on transient focal cerebral ischemia in rats PMID: 18067952
6. The results demonstrated that overexpression of TFPI gene might induce VSMC apoptosis in vitro through the mitochondrial pathway; meanwhile, IAP-1 expression is decreased. PMID: 18368493
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亚细胞定位：

Secreted.
组织特异性：

Most abundant in heart, lung, kidney, and aortic endothelial cells.
数据库链接：

KEGG: rno:29436

STRING: 10116.ENSRNOP00000006787

UniGene: Rn.15795

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.