Rat Thyroid-Peroxidase,TPO ELISA Kit

中文名称：

大鼠甲状腺过氧化物酶(TPO)酶联免疫试剂盒
货号：

CSB-E08352r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠甲状腺过氧化物酶（TPO）酶联免疫检测试剂盒（货号CSB-E08352r），采用竞争法原理实现血清、血浆及组织匀浆样本中TPO蛋白的定量分析。TPO是甲状腺激素合成的关键酶，催化碘化反应和酪氨酸偶联过程，其异常表达与自身免疫性甲状腺疾病密切相关，是研究甲状腺功能及免疫病理机制的重要靶点。试剂盒检测范围为2.5-40 ng/mL，覆盖大鼠样本中TPO的生理及病理浓度区间，实验全程约需4-5小时完成。每盒提供96孔预包被板、系列浓度标准品、生物素标记检测抗体及显色底物系统，操作时仅需将样本与标准品同步孵育后依次加入检测试剂，通过酶标仪读取450 nm吸光度值即可建立标准曲线并计算浓度。该试剂盒适用于甲状腺相关疾病的动物模型研究、环境毒理评估中甲状腺功能干扰物的筛选，以及甲状腺自身抗体作用机制等基础科研领域，实验前建议对组织样本进行充分匀浆处理，液体样本需离心去除颗粒物后分装冻存备用。
别名：

Tpo ELISA Kit; Thyroid peroxidase ELISA Kit; TPO ELISA Kit; EC 1.11.1.8 ELISA Kit
缩写：

TPO
Uniprot No.：

P14650
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue Homogenates
检测范围：

2.5 ng/mL-40ng/mL
灵敏度：

2.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	95
	Range %	84-99
1:4	Average %	90
	Range %	82-100
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of rat TPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-102
EDTA plasma (n=4)	94	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.160	0.154	0.157
20	0.269	0.265	0.267
10	0.499	0.512	0.506
5	1.043	1.007	1.025
2.5	2.051	2.037	2.044

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

引用文献

Sex Differences in the Production of SLC5A5, Thyroid Peroxidase, and Thyroid Hormones in Pubertal Rats Exposed to Endocrine Disruptor Yaglova NV.et al,Bull Exp Biol Med,2018

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功能：

Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).
亚细胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Peroxidase family, XPO subfamily
数据库链接：

STRING: 10116.ENSRNOP00000006526

UniGene: Rn.233736

Rat Thyroid-Peroxidase,TPO ELISA Kit

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