Rat Thyroglobulin,TG ELISA Kit

产品详情

产品描述：

CUSABIO定量检测大鼠血清、血浆及组织匀浆样本中甲状腺球蛋白（TG）的竞争法酶联免疫试剂盒（货号：CSB-E08240r），适用于科研领域对甲状腺功能及相关疾病的机制研究。甲状腺球蛋白是甲状腺滤泡细胞合成的特异性糖蛋白，作为甲状腺激素合成的前体物质，其水平变化可反映甲状腺代谢状态及病理损伤程度，是大鼠甲状腺炎、甲状腺肿或肿瘤模型研究的重要生物标志物。CUSABIO试剂盒采用竞争性ELISA原理，通过标准品与样本中TG竞争结合固相抗体实现定量分析，检测灵敏度覆盖10 ng/mL至160 ng/mL的线性范围，可精准检测低浓度样本。实验操作兼容血清、血浆（建议EDTA抗凝血）及组织匀浆（需离心去沉淀）三类样本，无需复杂前处理。该试剂盒适用于甲状腺相关药物的作用评估、环境毒素对甲状腺影响的机制研究，以及甲状腺疾病动物模型中病理进程的动态监测，为科研工作者提供稳定可靠的检测工具。
别名：

TgThyroglobulin ELISA kit; Tg ELISA kit
缩写：

TG
Uniprot No.：

P06882
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue Homogenates
检测范围：

10 ng/mL-160 ng/mL
灵敏度：

10 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat TG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-102
1:2	Average %	92
	Range %	86-99
1:4	Average %	95
	Range %	87-105
1:8	Average %	90
	Range %	83-102

回收率：

The recovery of rat TG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-100
EDTA plasma (n=4)	95	88-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Acts as a substrate for the production of iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). The synthesis of T3 and T4 involves iodination of selected tyrosine residues of TG/thyroglobulin followed by their oxidative coupling. Following TG re-internalization and lysosomal-mediated proteolysis, T3 and T4 are released from the polypeptide backbone leading to their secretion into the bloodstream. One dimer produces 7 thyroid hormone molecules.
相关疾病：

Defects in Tg are a cause of a form of hypothyroidism in rdw rat.
亚细胞定位：

Secreted.
蛋白家族：

Type-B carboxylesterase/lipase family
组织特异性：

Specifically expressed in the thyroid gland.
数据库链接：

STRING: 10116.ENSRNOP00000009241

UniGene: Rn.10429