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  4. Rat Tau Proteins ELISA kit

Rat Tau Proteins ELISA kit

文献引用 (7) 说明书下载
  • 中文名称：
    大鼠Tau蛋白酶联免疫试剂盒
  • 货号：
    CSB-E13729r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO专为大鼠样本设计的Tau蛋白酶联免疫检测试剂盒（货号：CSB-E13729r），采用双抗体夹心法定量检测血清、血浆及脑脊液（CSF）中的Tau蛋白浓度。Tau蛋白是一种神经元特异性微管相关蛋白，其异常磷酸化或聚集与神经退行性疾病机制研究密切相关，尤其在阿尔茨海默病、创伤性脑损伤等模型中常作为关键生物标志物。该试剂盒具备高灵敏度与特异性，检测范围为62.5-4000 pg/mL，可精准识别低丰度样本，适用于动态监测实验动物模型中Tau蛋白的表达变化。操作流程标准化，兼容多种生物样本类型，脑脊液的检测能力使其特别适用于神经系统疾病的基础研究、药物干预效果评估或病理进程分析。试剂盒提供预包被抗体及优化反应体系，实验全程无需额外配制核心试剂，3.5小时内即可完成检测，配套标准品及质控样本确保数据可靠性。本产品仅用于科学研究，为神经生物学、药理学及疾病机制探索提供高效工具。
  • 别名：
    Mapt ELISA Kit; Mtapt ELISA Kit; Tau ELISA Kit; Microtubule-associated protein tau ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament-tau ELISA Kit; PHF-tau ELISA Kit
  • 缩写：
    MAPT
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cerebrospinal fluid (CSF)
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat tau proteins in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    SampleSerum(n=4)
    1:5Average %98
    Range %95-101
    1:10Average %90
    Range %88-92
    1:20Average %85
    Range %81-89
    1:40Average %98
    Range %95-101
  • 回收率：
    The recovery of rat tau proteins spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10199-105
    EDTA plasma (n=4)8784-90
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    40002.694 2.678 2.686 2.523
    20002.104 2.156 2.130 1.967
    10001.330 1.356 1.343 1.180
    5000.825 0.836 0.831 0.668
    2500.552 0.568 0.560 0.397
    1250.359 0.368 0.364 0.201
    62.50.276 0.298 0.287 0.124
    00.167 0.159 0.163
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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相关问答

■ 常见问题解答
Q:

I want to know if CSB-E13729r detects Tau regardless of phosphorylation state?

A:
Thanks for your inquiry.
CSB-E13729r detect the total Tau, including the phosphorylated form.Pls let me know if you have any further questions. Thank you.

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靶点详情

  • 功能：
    Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
  • 亚细胞定位：
    Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cell projection, axon. Cell projection, dendrite. Secreted.
  • 组织特异性：
    Expressed in neurons. The larger forms (isoform tau-A and isoform tau-B) are preferentially expressed in the peripheral nervous system while the other are expressed in the central nervous system. Low amounts of the larger forms are also found in limited a
  • 数据库链接：

    STRING: 10116.ENSRNOP00000006856

    UniGene: Rn.2455

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