Rat Syndecan-2(SDC2) ELISA kit

Rat Syndecan-2(SDC2) ELISA kit

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中文名称：

大鼠多配体聚糖2(SDC2)酶联免疫试剂盒
货号：

CSB-EL020889RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠多配体聚糖2(SDC2)酶联免疫试剂盒（CSB-EL020889RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的SDC2含量。大鼠多配体聚糖2（SDC2）是一种跨膜蛋白聚糖，在多种癌症中表达升高，其研究机制涉及基因甲基化，与肿瘤生长、转移和分化密切相关。SDC2在结直肠癌等癌症的诊断和治疗中具有潜在应用价值。试剂盒检测范围为3.12 ng/mL-200 ng/mL，该产品适用于基础科研中探索SDC2在组织发育、细胞间相互作用中的功能机制，或用于评估疾病模型（如肿瘤、纤维化或感染性疾病）中SDC2的表达调控，为相关分子机制研究及药物干预效果评价提供可靠的定量分析工具，实验操作兼容多种生物样本类型，满足多场景科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Sdc2; Hspg1; Synd2; Syndecan-2; SYND2; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; CD antigen CD362
缩写：

SDC2
Uniprot No.：

P34900
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SDC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	93-105
1:2	Average %	96
	Range %	91-100
1:4	Average %	94
	Range %	89-98
1:8	Average %	85
	Range %	81-89

回收率：

The recovery of rat SDC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	96	90-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.562	2.463	2.513	2.388
100	2.113	1.994	2.054	1.929
50	1.520	1.491	1.506	1.381
25	1.053	1.044	1.049	0.924
12.5	0.614	0.623	0.619	0.494
6.25	0.361	0.370	0.366	0.241
3.12	0.239	0.243	0.241	0.116
0	0.124	0.125	0.125

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis.
基因功能参考文献：
1. postsynaptic SDC2 uses its ectodomain to interact with and facilitate dendritic filopodial targeting of FGF22, triggering presynaptic maturation. PMID: 27627962
2. Data suggest that the cytoplasmic domain of Sdc2 is involved in regulation of expression of MMP7 in colon carcinoma/adenocarcinoma cells; induction of MMP7 involves protein kinase C gamma-mediated FAK/ERK signaling. (Sdc2 = syndecan-2; MMP7 = matrix metalloproteinase-7; FAK = focal adhesion kinase 1) PMID: 28821612
3. Syndecan-2 and -4 form heterdimer and this interaction regulates syndecan-mediated cellular functions. PMID: 25979339
4. Data suggest that phenylalanine Phe(167) in the transmembrane domain of syndecan-2 endows the protein with specific functions. PMID: 25572401
5. Data suggest that Y-P30 can control the nuclear localization of Calcium/calmodulin-dependent serine kinase (CASK) in a syndecan (SDC) -2 and -3 -dependent manner. PMID: 24498267
6. syndecan-2 expression occurs in developmentally regulated patterns and syndecan-2 probably has different roles in adult and developing anterior pituitary glands. PMID: 23673414
7. pleiotrophin (PTN) and syndecan-2 and -3 as direct binding partners of Y-P30. PMID: 18599487
8. findings suggest that Sdc-2 plays an important role in microglial production of inflammatory cytokines, chemokines, and reactive oxygen species in hypoxic conditions PMID: 18803305
9. these results demonstrate a novel cooperation between syndecan-2 and integrin alpha2beta1 in adhesion-mediated cell migration and invasion. PMID: 19394307
10. Syndecan-2 plays a crucial role in the migratory potential of melanoma cells. PMID: 19641225
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亚细胞定位：

Membrane; Single-pass type I membrane protein.
蛋白家族：

Syndecan proteoglycan family
数据库链接：

KEGG: rno:25615

STRING: 10116.ENSRNOP00000007255

UniGene: Rn.11127

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.