Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

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中文名称：

大鼠雌激素硫酸转移酶(SULT1E1)酶联免疫试剂盒
货号：

CSB-E13472r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠雌激素硫酸转移酶(SULT1E1)酶联免疫试剂盒（CSB-E13472r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SULT1E1含量。SULT1E1 属于磺基转移酶家族。其背景在于参与体内多种内源性和外源性物质的磺化代谢。研究机制方面，主要是催化底物与 3'-磷酸腺苷 5'-磷酸硫酸（PAPS）发生磺化反应，改变底物活性、水溶性等，在激素调节、药物代谢等过程中有重要作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研场景中探索SULT1E1在激素代谢通路中的作用，例如评估卵巢、肝脏等组织中的酶活性变化，或分析药物干预、疾病模型下雌激素硫酸化水平波动。为生殖生物学、药理学及内分泌领域的基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Sult1e1 ELISA Kit; Ste ELISA Kit; Ste1 ELISA Kit; Sulfotransferase 1E1 ELISA Kit; ST1E1 ELISA Kit; EC 2.8.2.4 ELISA Kit; Estrogen sulfotransferase ELISA Kit; isoform 1 ELISA Kit; EST-1 ELISA Kit; Estrone sulfotransferase ELISA Kit; Sulfotransferase ELISA Kit; estrogen-preferring ELISA Kit
缩写：

SULT1E1
Uniprot No.：

P52844
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SULT1E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-93
1:2	Average %	108
	Range %	99-111
1:4	Average %	92
	Range %	87-97
1:8	Average %	99
	Range %	92-103

回收率：

The recovery of rat SULT1E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	87-97
EDTA plasma (n=4)	99	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.488	2.377	2.433	2.300
5	2.075	2.045	2.060	1.927
2.5	1.412	1.389	1.401	1.268
1.25	0.916	0.898	0.907	0.774
0.625	0.574	0.564	0.569	0.436
0.312	0.353	0.367	0.360	0.227
0.156	0.267	0.260	0.264	0.131
0	0.134	0.132	0.133

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine.
亚细胞定位：

Cytoplasm, cytosol.
蛋白家族：

Sulfotransferase 1 family
数据库链接：

UniGene: Rn.32104

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.