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  4. Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit

Rat Steroid 5-Alpha-Reductase 1 (SRD5A1) ELISA Kit

文献引用 (5) 说明书下载
  • 中文名称：
    大鼠类固醇5α还原酶1 (SRD5A1)酶联免疫试剂盒
  • 货号：
    CSB-E14053r
  • 规格：
    96T/48T
  • 价格：
    ￥3900/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠类固醇5α还原酶1 (SRD5A1)酶联免疫试剂盒（CSB-E14053r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的SRD5A1含量。SRD5A1即5α-还原酶1型，在多种组织中表达。它能将睾酮转化为活性更强的双氢睾酮。对其研究机制主要围绕调节雄激素活性展开，在前列腺疾病、脱发等病症的发生发展中扮演角色，为相关治疗药物研发提供了重要靶点。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于实验室研究中对SRD5A1表达水平的动态监测，如内分泌代谢研究、药物干预实验或疾病模型构建中酶活性变化的定量分析。为探索SRD5A1在生理及病理过程中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Srd5a1; 3-oxo-5-alpha-steroid 4-dehydrogenase 1; SR type 1; Steroid 5-alpha-reductase 1; S5AR 1
  • 缩写：
    SRD5A1
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates
  • 检测范围：
    15.6 pg/mL-1000 pg/mL
  • 灵敏度：
    3.9 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Neuroscience
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat SRD5A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　SampleSerum(n=4)
    1:5Average %100
    Range %95-105
    1:10Average %96
    Range %90-102
    1:20Average %94
    Range %88-99
    1:40Average %90
    Range %85-95
  • 回收率：
    The recovery of rat SRD5A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample TypeAverage % RecoveryRange
    Serum (n=5) 10198-105
    EDTA plasma (n=4)9590-100
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/mlOD1OD2AverageCorrected
    10002.762 2.803 2.783 2.589
    5002.117 2.104 2.111 1.917
    2501.598 1.583 1.591 1.397
    1251.103 1.136 1.120 0.926
    62.50.652 0.672 0.662 0.468
    31.250.416 0.426 0.421 0.227
    15.60.341 0.351 0.346 0.152
    00.193 0.195 0.194 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

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Srd5a1 Proteins

靶点详情

  • 功能：
    Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.
  • 基因功能参考文献：
    1. Ziram inhibited SRD5A1 and AKR1C14 activities, thus possibly interfering with neurosteroid production in rats. PMID: 28707553
    2. Exposure of middle-aged rats to environmental enrichment increased mRNA levels of 5al;pha-reductase-1, 3alpha-HSD and cytochrome P450 17alpha-hydroxylase/c17,20-lyase and decreased the methylation state of the 5alpha-reductase-1 gene PMID: 26021641
    3. Low Srd5a1 expression is associated with benign prostatic hypertrophy. PMID: 23405234
    4. Changes in the expression of steroid 5-alpha reductase isoenzymes in the prostate in response to environmental stress. PMID: 23122426
    5. Tesosterone concentrations during postnatal sexual differentiation of the rat central nervous system, among other sex-dependent factors, influence brain levels of 5alpha-Reductase isozymes in adulthood PMID: 22131296
    6. Elevated levels of 5alpha-reduced androgens seen in hyperinsulinemic conditions might be explained on the basis of a stimulatory effect of insulin on 5alpha-reductase in granulosa cells. PMID: 20810561
    7. Thus, the present results indicate that the vitamin E-induced regulation of SRD5A1 and GCLM in rat liver is not directly mediated by changes in promoter DNA methylation. PMID: 20447326
    8. 5 alpha-R1 immunostaining was detected mainly in the white matter (Wm). PMID: 15305365
    9. great sexual dimorphism in the central nervous system (CNS) with respect to both 5alpha-reductase isozymes, and suggest a crucial role of dihydrotestosterone in the sexual dimorphism of the CNS in the female PMID: 16595696
    10. Our results demonstrate that both 5alpha-R isozymes are significantly higher in prefrontal cortex of male rats after acute swim stress in comparison with control rats PMID: 17826869
    11. Our results suggest that 5AR inhibitors elicit antipsychotic-like effects in animals and may be proposed as a putative novel target in the management of psychotic disorders. PMID: 18354385

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  • 亚细胞定位：
    Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Steroid 5-alpha reductase family
  • 组织特异性：
    Liver and prostate (at a low level).
  • 数据库链接：

    KEGG: rno:24950

    STRING: 10116.ENSRNOP00000023659

    UniGene: Rn.4620

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |