Rat Runt-related transcription factor 2(RUNX2) ELISA kit

Rat Runt-related transcription factor 2(RUNX2) ELISA kit

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中文名称：

大鼠Runt相关转录因子2(RUNX2)酶联免疫试剂盒
货号：

CSB-EL020594RA
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

大鼠Runt相关转录因子2(RUNX2)酶联免疫试剂盒（CSB-EL020594RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的RUNX2含量。RUNX2是重要的转录因子，在骨骼发育和骨代谢中起关键作用。其能调控成骨细胞分化和软骨细胞成熟。研究机制主要聚焦于它与多种信号通路相互作用，如BMP、Wnt等，进而对骨形成相关基因表达进行调节。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于体外科研实验中探索骨骼发育机制、骨代谢调控、骨质疏松模型评估、骨再生修复研究以及干细胞向成骨细胞分化的动态监测等领域。为骨生物学、骨组织工程及骨疾病机制研究提供可靠的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Runx2 ELISA Kit; Cbfa1 ELISA Kit; Osf2 ELISA Kit; Runt-related transcription factor 2 ELISA Kit; Core-binding factor subunit alpha-1 ELISA Kit; CBF-alpha-1 ELISA Kit; Osteoblast-specific transcription factor 2 ELISA Kit; OSF-2 ELISA Kit; Fragments ELISA Kit
缩写：

RUNX2
Uniprot No.：

Q9Z2J9
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates, cell lysates
检测范围：

3.12 ng/mL-200 ng/mL
灵敏度：

0.78 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RUNX2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	91-103
1:2	Average %	102
	Range %	94-107
1:4	Average %	88
	Range %	83-91
1:8	Average %	94
	Range %	84-98

回收率：

The recovery of rat RUNX2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	93-107
EDTA plasma (n=4)	98	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.551	2.452	2.502	2.322
100	2.126	1.996	2.061	1.881
50	1.359	1.341	1.350	1.170
25	0.793	0.818	0.806	0.626
12.5	0.474	0.481	0.478	0.298
6.25	0.395	0.403	0.399	0.219
3.12	0.266	0.257	0.262	0.082
0	0.182	0.178	0.180

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Tofu-Incorporated Hydrogels for Potential Bone Regeneration K Huang,ACS Biomater. Sci. Eng,2020

功能：

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).
亚细胞定位：

Nucleus.
数据库链接：

STRING: 10116.ENSRNOP00000057733

UniGene: Rn.83672

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.