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中文名称：大鼠类风湿因子(RF)酶联免疫试剂盒
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货号：CSB-E13666r
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规格：96T/48T
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价格：￥3600/￥2500
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其他：
产品详情
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产品描述：CUSABIO大鼠类风湿因子（RF）酶联免疫检测试剂盒（货号：CSB-E13666r），基于双抗体夹心法原理，可定量检测血清或血浆样本中15.6-1000 mIU/mL浓度范围的RF水平。类风湿因子作为自身抗体IgG的Fc段结合蛋白，在类风湿性关节炎、干燥综合征等自身免疫疾病的研究中具有重要价值，其定量检测可为疾病模型构建、病理机制探索及药物干预效果评估提供数据支持。试剂盒采用预包被特异性抗体的96孔板，实验全程约需4小时，包含标准品、检测抗体及显色底物等全套组分，操作流程标准化且重复性良好。该产品适用于科研场景下大鼠血清或血浆样本的RF水平检测，如关节炎动物模型的炎症程度分析、免疫调节剂筛选实验或基础医学研究中自身免疫反应动态监测，为研究者提供稳定可靠的实验工具。
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别名：N/A
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缩写：RF
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种属：Rattus norvegicus (Rat)
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样本类型：serum, plasma
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检测范围：15.6 mIU/mL-1000 mIU/mL
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灵敏度：3.9 mIU/mL
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反应时间：1-5h
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样本体积：50-100ul
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检测波长：450 nm
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研究领域：Immunology
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测定原理：quantitative
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测定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat RF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:5 Average % 104 Range % 96-108 1:10 Average % 94 Range % 91-98 1:20 Average % 102 Range % 98-106 1:40 Average % 88 Range % 82-96 -
回收率：
The recovery of rat RF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 83-95 EDTA plasma (n=4) 99 92-107 -
标准曲线：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
mIU/ml OD1 OD2 Average Corrected 1000 2.646 2.545 2.596 2.443 500 1.975 2.076 2.026 1.873 250 1.284 1.308 1.296 1.143 125 0.751 0.762 0.757 0.604 62.5 0.512 0.501 0.507 0.354 31.2 0.353 0.342 0.348 0.195 15.6 0.285 0.281 0.283 0.130 0 0.153 0.152 0.153 -
数据处理：
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货期：3-5 working days
引用文献
- Norisoboldine Reduces Arthritis Severity by Attenuating Inflammation, Oxidative Stress, and Extracellular Matrix Degradation in a Rat Model of Rheumatoid Arthritis Y Wang, X Zhao, J Wang, X Zhu,Journal of Inflammation Research,2024
- Visnagin alleviates rheumatoid arthritis via its potential inhibitory impact on malate dehydrogenase enzyme: in silico, in vitro, and in vivo studies AA Khamis, AH Sharshar, TM Mohamed,Genes & Nutrition,2024
- Investigating the therapeutic potential of sinomenine in rheumatoid arthritis: anti-inflammatory, antioxidant, and immunomodulatory mechanisms J Li,Naunyn-Schmiedeberg's archives of pharmacology,2023
- Cerastes snake venom as a promising approach in the management of complete Freund's adjuvant-induced rheumatoid arthritis in rats: Involvement of RANKL and JAK/STAT pathway NS Soliman,Journal of ethnopharmacology,2023
- Anti-arthritic activity of solvent extracts of the bulbs of Crinum pedunculatum R.Br. P Doe,Pharmaceutical Sciences Asia,2022
- CS12192, a Novel JAK3/JAK1/TBK1 Inhibitor, Synergistically Enhances the Anti-Inflammation Effect of Methotrexate in a Rat Model of Rheumatoid Arthritis Z Fang,International journal of molecular sciences,2022
- Diosmin and Trolox Have Anti-Arthritic, Anti-Inflammatory and Antioxidant Potencies in Complete Freund’s Adjuvant-Induced Arthritic Male Wistar Rats: Roles of NF-κB, iNOS, Nrf2 and MMPs HH Shaaban,Antioxidants,2022
- Leurieus quinquestriatus scorpion venom ameliorates adjuvant-induced arthritis in rats: Modulating JAK/STAT/RANKL signal transduction pathway NS Soliman,International immunopharmacology,2022
- Anti-inflammatory effects of evening primrose and extra virgin olive oil on arthritc rats BM Mahmoud,Advances in Animal and Veterinary Sciences,2021
- The Enhanced Beneficial Effect of Extra Virgin Olive Oil Over Evening Primrose Oil on Oxidative Stress, and Liver Function in Male Arthritic Rats S Zhang,Journal of Pharmaceutical Research International,2021
- TDZD-8 Suppresses Rheumatoid Arthritis Induced by Collagen Type II in Rats: Potential Role of Apoptosis and Inflammation AF Dawood,International Journal of Morphology,2021
- Activation of activin/Smad2 and 3 signaling pathway and the potential involvement of endothelial‑mesenchymal transition in the valvular damage due to rheumatic heart disease S Xian,Molecular Medicine Reports,2020
- Inhibition of GSK3β protects against collagen type II┸\induced arthritis associated with a decrease in synovial leukocyte infiltration and inhibition of endoplasmic … NM Alzamil,Clin Exp Pharmacol Physiol,2020
- Inhibition of glycogen synthase kinase-3β protects against collagen type II-induced arthritis associated with the inhibition of miR155/24 and inflammation and … AF Dawood,Arch. Physiol. Biochem,2020
- A novel mPGES-1 inhibitor alleviates inflammatory responses by downregulating PGE2 in experimental models Lee HH, et al,Prostaglandins & Other Lipid Mediators,2019
- Effect of mandarin peel extract on experimentally induced arthritis in male rats Sakr HI,ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY,2019
- Ulva lactuca hydroethanolic extract suppresses experimental arthritis via its anti-inflammatory and antioxidant activities Osama M. Ahmed.et al,Beni-Suef University Journal of Basic and Applied Sciences,2017
- Standardized ethyl acetate fraction from the roots of Brassica rapa attenuates the experimental arthritis by down regulating inflammatory responses and inhibiting NF-kB activation. Shin JS et al,Food Chem Toxicol,2014
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