Rat Resistin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO针对大鼠抵抗素（Resistin）定量检测的酶联免疫试剂盒（货号：CSB-E06885r），采用双抗体夹心法原理设计，可精准检测血清、血浆及组织匀浆样本中的Resistin含量，检测范围为125至8000 pg/mL。抵抗素是一种由脂肪细胞分泌的多肽类激素，参与调节糖脂代谢及胰岛素敏感性，其表达水平与肥胖、胰岛素抵抗等代谢性疾病密切相关。该试剂盒通过高亲和力抗体实现特异性捕获与信号放大，操作流程包含样本预处理、孵育、洗涤及显色等标准化步骤，适配常规实验室设备。适用于科研领域中对大鼠代谢疾病模型（如2型糖尿病、肥胖症）的机制研究、药物干预效果评估或Resistin表达调控实验，可通过检测不同生理病理状态下血清、组织等样本的Resistin水平，揭示其在能量代谢与炎症反应中的作用。试剂盒提供完整组分及操作说明书，确保实验稳定性和重复性，满足基础研究与生物标志物探索需求。
缩写：

Resistin
Uniprot No.：

Q8K4J7
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 pg/mL-8000 pg/mL
灵敏度：

31.25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat resistin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	96
	Range %	91-101
1:4	Average %	95
	Range %	90-101
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of rat resistin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-94
EDTA plasma (n=4)	99	95-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.