Rat Renalase/ Monoamine Oxidase-C(RNLS/ MAO-C)ELISA Kit

产品详情

产品描述：

CUSABIO针对大鼠肾胺酶（RNLS/MAO-C）的定量检测试剂盒，适用于血清、血浆及组织匀浆样本的科研分析。肾胺酶是一种重要的含铜胺氧化酶，参与调节血压、氧化应激及炎症反应等生理过程，其表达水平与肾脏功能异常、心血管疾病及代谢综合征等病理机制密切相关。试剂盒采用双抗体夹心法原理，通过预包被特异性抗体捕获目标蛋白，并结合酶标二抗进行信号放大，检测范围覆盖62.5-4000 ng/mL的线性区间，可精准检测样本中RNLS的浓度变化。该试剂盒适用于基础医学研究，如探索高血压、糖尿病肾病等动物模型中肾胺酶的表达调控机制，或用于药理学研究评估药物对肾脏氧化应激通路的影响。实验操作兼容常规实验室设备，提供标准品及预包被96孔板，确保实验流程高效便捷。本产品仅限科学研究使用，不涉及任何临床诊断用途。
别名：

Rnls ELISA Kit; Renalase ELISA Kit; EC 1.6.3.5 ELISA Kit; Monoamine oxidase-C ELISA Kit; MAO-C ELISA Kit; alpha-NAD(P)H oxidase/anomerase ELISA Kit
缩写：

C10orf59
Uniprot No.：

Q5U2W9
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 ng/mL-4000 ng/mL
灵敏度：

15.6 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat RNLS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	106
	Range %	101-113
1:4	Average %	94
	Range %	90-98
1:8	Average %	89
	Range %	84-93

回收率：

The recovery of rat RNLS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-91
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Catalyzes the oxidation of the less abundant 1,2-dihydro-beta-NAD(P) and 1,6-dihydro-beta-NAD(P) to form beta-NAD(P)(+). The enzyme hormone is secreted by the kidney, and circulates in blood and modulates cardiac function and systemic blood pressure. Lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis.
基因功能参考文献：
1. The brain and peripheral tissues renalase mRNA levels demonstrate opposite trends, which are obviously crucial for impaired regulation of blood pressure in spontaneously hypertensive rats. PMID: 24113803
2. exercise differentially regulates renalase expression in skeletal muscle and kidney in rats PMID: 24366404
3. Renalase deficiency occurs in salt-sensitive Dahl rats as they develop hypertension[review] PMID: 17565281
亚细胞定位：

Secreted.
蛋白家族：

Renalase family
数据库链接：

KEGG: rno:361751

STRING: 10116.ENSRNOP00000028098

UniGene: Rn.15885