Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit

产品详情

产品评价

靶点详情

产品详情

产品评价

相关产品

靶点详情

Most popular with customers

产品中心

信号通路

技术信息

联系信息

中文名称：

大鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒
货号：

CSB-E12630r
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情
产品评价
相关产品
靶点详情

产品描述：

大鼠肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒（CSB-E12630r）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPB含量。SP-B是肺表面活性物质的重要组成部分，参与调节肺泡表面张力，维持呼吸功能。其研究机制涉及炎症反应、氧化应激，与肺发育障碍密切相关。近年来，深度学习等技术在药物-靶标相互作用预测方面取得进展，为SP-B相关疾病研究提供新思路。试剂盒检测范围为3.12 pg/mL-200 pg/mL，可广泛用于基础科研中肺部疾病机制研究、药物干预效果评估或动物模型（如肺损伤、肺纤维化及新生儿呼吸窘迫综合征）的SP - B动态监测，为探索SP - B在呼吸生理及病理过程中的调控作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写：

SFTPB
Uniprot No.：

P22355
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	85-100
1:2000	Average %	94
	Range %	87-98
1:4000	Average %	90
	Range %	85-96
1:8000	Average %	94
	Range %	90-98

回收率：

The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	91	85-97

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.470	2.589	2.530	2.377
100	2.115	2.124	2.120	1.967
50	1.713	1.726	1.720	1.567
25	1.315	1.356	1.336	1.183
12.5	0.985	0.971	0.978	0.825
6.25	0.532	0.541	0.537	0.384
3.12	0.378	0.389	0.384	0.231
0	0.152	0.154	0.153

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
基因功能参考文献：
1. SP-B is present on the surface of exocytosed surfactant particles. Antibodies against SP-B stabilize the released particles when contacting the air-liquid interface, precluding their transformation and the formation of surface films. SP-B acts as an essential, preformed molecular key in the initial stages of surfactant unpacking and surface film formation. PMID: 27155084
2. Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats PMID: 26196539
3. The role of the SP-B NH(2) terminus in the formation and stability of cylindrical structures, specifically the distribution of lipid stack height, width, and density with four SP-B truncation peptides, was studied. PMID: 20023175
4. VEGF increased expression of SP-b mRNA in preterm rat lung. PMID: 17267143
5. alterations of surfactant are a consequence of the ventilation strategy that impair the biophysical activity of this material and thereby contribute directly to lung dysfunction over time PMID: 18344412
显示更多

收起更多
亚细胞定位：

Secreted, extracellular space, surface film.
数据库链接：

KEGG: rno:192155

STRING: 10116.ENSRNOP00000014505

UniGene: Rn.1952

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.