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  4. Rat Protein S100-A6(S100A6) ELISA kit

Rat Protein S100-A6(S100A6) ELISA kit

文献引用 (2) 说明书下载
  • 中文名称：
    大鼠S100-A6蛋白(S100A6)酶联免疫试剂盒
  • 货号：
    CSB-EL020634RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    CUSABIO定量检测大鼠血清、血浆、组织匀浆或细胞裂解液中S100-A6蛋白（S100A6）的酶联免疫试剂盒，采用双抗体夹心法原理，具有高灵敏度和特异性。S100A6属于S100钙结合蛋白家族，广泛参与细胞周期调控、分化、迁移及胞内信号转导等过程，其表达异常与肿瘤发生发展、神经退行性疾病及炎症反应等病理机制相关。试剂盒检测范围覆盖2.4 ng/mL至150 ng/mL的宽线性范围，适用于多种样本类型的定量分析。实验操作基于预包被特异性抗体的酶标板，通过标准品梯度稀释建立标准曲线，结合生物素标记检测抗体与链霉亲和素-HRP显色系统，实现目标蛋白的精准定量。该试剂盒可广泛应用于神经生物学、肿瘤机制研究及炎症反应等领域的科研实验，例如在动物模型中分析S100A6在不同疾病状态下的动态表达水平，或探究其在细胞应激反应中的调节作用。本产品仅适用于科学研究，操作时需严格按照说明书进行样本预处理和实验流程。
  • 别名：
    S100A6 ELISA Kit; Protein S100-A6 ELISA Kit; Calcyclin ELISA Kit; Lung 10 kDa protein ELISA Kit; S100 calcium-binding protein A6 ELISA Kit
  • 缩写：
    S100A6
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates, cell lysates
  • 检测范围：
    2.4 ng/mL-150 ng/mL
  • 灵敏度：
    0.6 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Epigenetics and Nuclear Signaling
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat S100A6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 91-99
    1:2 Average % 84
    Range % 80-88
    1:4 Average % 107
    Range % 103-110
    1:8 Average % 112
    Range % 109-115
  • 回收率：
    The recovery of rat S100A6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 83 80-86
    EDTA plasma (n=4) 95 91-99
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    150 2.700 2.765 2.733 2.570
    75 1.985 1.935 1.960 1.797
    37.5 1.341 1.320 1.331 1.168
    18.75 0.870 0.868 0.869 0.706
    9.4 0.526 0.541 0.534 0.371
    4.7 0.395 0.382 0.389 0.226
    2.4 0.247 0.235 0.241 0.078
    0 0.162 0.164 0.163 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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S100a6 Proteins

靶点详情

  • 功能：
    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.
  • 亚细胞定位：
    Nucleus envelope. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    S-100 family
  • 数据库链接：

    KEGG: ocu:100348755

    STRING: 9986.ENSOCUP00000017711

    UniGene: Ocu.1714

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九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |