Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 试剂盒
  3. 酶联免疫试剂盒
  4. Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

Rat Prostaglandin-H2 D-isomerase(PTGDS) ELISA kit

文献引用 (1) 说明书下载
  • 中文名称：
    大鼠前列腺素-H2 D-异构酶(PTGDS)酶联免疫试剂盒
  • 货号：
    CSB-EL018969RA
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    大鼠前列腺素-H2 D-异构酶(PTGDS)酶联免疫试剂盒（CSB-EL018969RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）、组织匀浆样本中的PTGDS含量。PTGDS即前列腺素D合成酶，在多种生理和病理过程中发挥重要作用。其主要催化花生四烯酸代谢生成前列腺素D₂。在研究机制方面，它参与睡眠调节、炎症反应等过程，是神经炎症、疼痛等疾病潜在治疗靶点，相关研究在持续深入。试剂盒检测范围为9.37 ng/mL-600 ng/mL，该产品适用于科研领域中对前列腺素代谢通路、神经退行性疾病机制、炎症相关信号传导等方向的研究，支持多种生物样本类型的并行检测，为体外实验提供标准化分析工具。实验操作便捷，配套试剂完整，适用于实验室常规检测及高通量筛选场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Ptgds ELISA Kit; Prostaglandin-H2 D-isomerase ELISA Kit; EC 5.3.99.2 ELISA Kit; Glutathione-independent PGD synthase ELISA Kit; Lipocalin-type prostaglandin-D synthase ELISA Kit; Prostaglandin-D2 synthase ELISA Kit; PGD2 synthase ELISA Kit; PGDS ELISA Kit; PGDS2 ELISA Kit
  • 缩写：
    PTGDS
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, cerebrospinal fluid (CSF), tissue homogenates
  • 检测范围：
    9.37 ng/mL-600 ng/mL
  • 灵敏度：
    2.34 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Metabolism
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of rat PTGDS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 95
    Range % 90-100
    1:2 Average % 90
    Range % 85-94
    1:4 Average % 85
    Range % 80-89
    1:8 Average % 102
    Range % 98-107
  • 回收率：
    The recovery of rat PTGDS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 94 90-98
    EDTA plasma (n=4) 87 82-91
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    600 2.601 2.495 2.548 2.345
    300 2.004 1.916 1.960 1.757
    150 1.295 1.328 1.312 1.109
    75 0.817 0.860 0.839 0.636
    37.5 0.479 0.452 0.466 0.263
    18.75 0.352 0.339 0.346 0.143
    9.37 0.268 0.281 0.275 0.072
    0 0.199 0.207 0.203 　
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

引用文献

产品评价

相关产品

Ptgds Proteins

靶点详情

  • 功能：
    Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophobic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Involved in PLA2G3-dependent maturation of mast cells. PLA2G3 is secreted by immature mast cells and acts on nearby fibroblasts upstream to PTDGS to synthesize PGD2, which in turn promotes mast cell maturation and degranulation via PTGDR.
  • 基因功能参考文献：
    1. HIF-1alpha-l-PGDS-PPARgamma regulates hypoxia-induced ANP secretion in beating rat atria PMID: 29287795
    2. Lipocalin-type prostaglandin synthase (L-PGDS) may have a role in sleep and chronic intermittent hypoxia PMID: 28025147
    3. Anorectic tumor-bearing rats do not display the changes in ventral striatal prostaglandin D synthase expression observed in food-restricted weight-matched animals. PMID: 21334429
    4. Gene expression is regulated by Hes-1 through E-box and interleukin-1 beta via two NF-kappa B elements in rat leptomeningeal cells. PMID: 12488457
    5. study showed that L-PGDS was strikingly expressed in the caput epididymidis, while a moderate to weak expression was observed in the corpus and cauda epididymidis PMID: 16730417
    6. Prostaglandin D synthase and prostacyclin synthase are present and could be regulated by steroids in the rat uterus during pregnancy. Endometrium could be significant source of PGD(2) and PGI(2) at specific times during pregnancy. PMID: 17951541
    7. These results suggested that the removal of the disulfide bond slightly increased the affinities of L-PGDS for ligand binding by changing the hydrophobic regions. PMID: 18590794
    8. This cardioprotective effect of dexamethasone was markedly reduced in L-PGDS-deficient mice PMID: 19451694

    显示更多

    收起更多

  • 亚细胞定位：
    Rough endoplasmic reticulum. Nucleus membrane. Golgi apparatus. Cytoplasm, perinuclear region. Secreted.
  • 蛋白家族：
    Calycin superfamily, Lipocalin family
  • 组织特异性：
    Abundant in the brain and CNS, where it is expressed in tissues of the blood-brain barrier and secreted into the cerebro-spinal fluid. In the eye, it is expressed in the pigmented epithelium of the retina and the nonpigmented epithelium of the iris and ci
  • 数据库链接：

    KEGG: rno:25526

    STRING: 10116.ENSRNOP00000020926

    UniGene: Rn.11400

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |