Rat Platelet Factor 4,PF-4 ELISA Kit

Rat Platelet Factor 4,PF-4 ELISA Kit

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中文名称：

大鼠血小板因子4(PF-4/CXCL4)酶联免疫试剂盒
货号：

CSB-E07885r
规格：

96T/48T
价格：

￥3900/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO大鼠血小板因子4（PF-4/CXCL4）酶联免疫检测试剂盒（货号：CSB-E07885r），采用定量夹心法原理设计，可精准检测血清、血浆及组织匀浆样本中PF-4/CXCL4的含量，检测范围覆盖0.156 μg/mL至10 μg/mL。PF-4/CXCL4是血小板α颗粒释放的关键趋化因子，参与炎症反应、血栓形成及组织修复等生理病理过程，在心血管疾病和免疫调节研究中具有重要价值。本试剂盒基于双抗体夹心原理，通过预包被高特异性抗体捕获目标蛋白，结合标记抗体实现信号放大，具有高灵敏度和重复性，操作流程标准化，适用于体外实验中对PF-4/CXCL4表达水平的定量分析。科研人员可将其应用于血小板活化机制、血栓相关疾病模型或炎症微环境等研究领域，为探索PF-4/CXCL4在病理生理过程中的调控作用提供可靠工具。试剂盒兼容多种生物样本类型，满足实验室基础研究及药物开发中的靶点验证需求。
别名：

Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit
缩写：

PF4
Uniprot No.：

P06765
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 μg/mL-10 μg/mL
灵敏度：

0.039 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-97
1:2	Average %	95
	Range %	88-99
1:4	Average %	95
	Range %	91-102
1:8	Average %	95
	Range %	87-104

回收率：

The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-99
EDTA plasma (n=4)	97	89-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

μg/ml	OD1	OD2	Average	Corrected
10	2.684	2.617	2.651	2.463
5	2.204	2.186	2.195	2.007
2.5	1.563	1.608	1.586	1.398
1.25	0.881	0.899	0.890	0.702
0.625	0.480	0.491	0.486	0.298
0.312	0.345	0.337	0.341	0.153
0.156	0.247	0.250	0.249	0.061
0	0.185	0.190	0.188

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Influence of brush length of PVP chains immobilized on silicon wafers on their blood compatibility Shengqiang Nie.et al,Polymers for Advanced Technologies,2017

功能：

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.
基因功能参考文献：
1. identified the informative Chronic Liver Allograft Dysfunction (CLAD)-associated genes; CXCL4, CXCR3, EGFR, JAK2, STAT3, and Collagen IV were associated with CLAD pathogenesis; validated that CXCL4 is upstream of these informative genes in the isolated hepatic stellate cells PMID: 28053995
2. A carboxyl-terminal fragment of this protein effects a rapid histamine release from rat peritoneal mast cells. PMID: 12383857
3. this study identified a novel regulatory element,TME(tandem repeat of MEIS1 binding element)in the PF4 promoter and demonstrated that MEIS1/PBX complexes bind to the TME PMID: 12609849
4. PF4 promoters are transactivated by USF1 and 2 which may play an important role in megakaryocytic gene expression PMID: 15187018
5. The levels of PF-4 (as a platelet marker) mRNA were significantly higher in FeCl(2)-VT (2(-DeltaDeltaCT)=7.8) than in stasis-VT (2(-DeltaDeltaCT)=4.2, p<0.05). PMID: 16797058
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亚细胞定位：

Secreted.
蛋白家族：

Intercrine alpha (chemokine CxC) family
数据库链接：

KEGG: rno:360918

STRING: 10116.ENSRNOP00000032276

UniGene: Rn.6800

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.