Rat Plasma kallikrein(KLKB1) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

大鼠血浆激肽释放酶(KLKB1)酶联免疫试剂盒（CSB-EL012461RA）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的KLKB1含量。KLKB1 是一个重要靶点。它参与多种生理病理过程，如凝血与纤溶系统调节。在研究机制上，其活性变化可影响血管通透性、炎症反应等。对 KLKB1 的深入研究有助于揭示相关疾病发病机制，为开发针对性药物和治疗策略提供方向。试剂盒检测范围为7.8 ng/mL-500 ng/mL，适用于心血管疾病、代谢调控及炎症相关机制的基础研究，也可用于药理学实验中药物对 KLKB1 表达水平影响的评估。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Klkb1 ELISA Kit; Klk3 ELISA Kit; PkPlasma kallikrein ELISA Kit; EC 3.4.21.34 ELISA Kit; Fletcher factor ELISA Kit; Kininogenin ELISA Kit; Plasma prekallikrein) [Cleaved into: Plasma kallikrein heavy chain; Plasma kallikrein light chain] ELISA Kit
缩写：

KLKB1
Uniprot No.：

P14272
种属：

Rattus norvegicus (Rat)
样本类型：

serum, plasma, tissue homogenates
检测范围：

7.8 ng/mL-500 ng/mL
灵敏度：

1.95 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat KLKB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	91
	Range %	86-95
1:400	Average %	103
	Range %	99-105
1:800	Average %	91
	Range %	85-97
1:1600	Average %	96
	Range %	91-99

回收率：

The recovery of rat KLKB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	95-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
亚细胞定位：

Secreted.
蛋白家族：

Peptidase S1 family, Plasma kallikrein subfamily
数据库链接：

STRING: 10116.ENSRNOP00000019237

UniGene: Rn.9880